α-Cat-RNAi knockdown and distribution and activity of M region deletion constructs. (A) α-Cat-RNAi expressed by mat-GAL4 causes loss of any detectable cortical α-Cat in stage 8 embryos. Yellow scale bar, 100 μm; white scale bars, 10 μm. (B) Quantification of average levels of cortical α-Cat per embryo. Significance calculated by Mann-Whitney two-tailed t test, **** = P < 0.0001. Bar height, mean; error bars, SD; n = number of embryos (e). (C) Immunostaining and localization of HA-tagged α-Cat constructs expressed in embryos with α-Cat-RNAi background. Scale bar, 10 μm. (D and E) Close-ups of the mesoderm (C) and lateral ectoderm (D) of α-Cat-RNAi expressing embryos, showing membrane tethers and loss of AJs. Cells lose contact apically while basal membranes remain in contact. Scale bar, 10 μm. (F) Example images of terminal cuticle phenotypes of α-Cat-RNAi α-CatX embryos. Scale bar, 100 μm.
Figure S2.

α-Cat-RNAi knockdown and distribution and activity of M region deletion constructs. (A) α-Cat-RNAi expressed by mat-GAL4 causes loss of any detectable cortical α-Cat in stage 8 embryos. Yellow scale bar, 100 μm; white scale bars, 10 μm. (B) Quantification of average levels of cortical α-Cat per embryo. Significance calculated by Mann-Whitney two-tailed t test, **** = P < 0.0001. Bar height, mean; error bars, SD; n = number of embryos (e). (C) Immunostaining and localization of HA-tagged α-Cat constructs expressed in embryos with α-Cat-RNAi background. Scale bar, 10 μm. (D and E) Close-ups of the mesoderm (C) and lateral ectoderm (D) of α-Cat-RNAi expressing embryos, showing membrane tethers and loss of AJs. Cells lose contact apically while basal membranes remain in contact. Scale bar, 10 μm. (F) Example images of terminal cuticle phenotypes of α-Cat-RNAi α-CatX embryos. Scale bar, 100 μm.

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