Supporting data pertaining to nuclear basket behavior during S. pombe mitosis. (A–F) Montages of strains with GFP-tagged nucleoporins during mitosis: (A) SpNup60-GFP (fySLJ745); (B) SpNup61-GFP (fySLJ870); (C) SpNup124-GFP (fySLJ1018); (D) SpNup211-GFP (fySLJ1019); (E) SpAlm1-GFP (fySLJ867); and (F) SpPom34-GFP (fySLJ537). Cells were staged according to the timing of SPB separation as visualized using SpPpc89-mCherry (not shown). The white arrowheads in the “Fire LUT” images denote nuclei after mitotic SPB separation, at the time point when SpNup60-SpNup61 detachment occurs. The individual (gray) and mean (black) nucleoporin intensity profiles measured on single z-slices are shown below for the indicated number of individual nuclei (n), 10 min before and 5 min after SPB separation. Scale bars, 3 μm.
Figure S9.

Supporting data pertaining to nuclear basket behavior during S. pombe mitosis. (A–F) Montages of strains with GFP-tagged nucleoporins during mitosis: (A) SpNup60-GFP (fySLJ745); (B) SpNup61-GFP (fySLJ870); (C) SpNup124-GFP (fySLJ1018); (D) SpNup211-GFP (fySLJ1019); (E) SpAlm1-GFP (fySLJ867); and (F) SpPom34-GFP (fySLJ537). Cells were staged according to the timing of SPB separation as visualized using SpPpc89-mCherry (not shown). The white arrowheads in the “Fire LUT” images denote nuclei after mitotic SPB separation, at the time point when SpNup60-SpNup61 detachment occurs. The individual (gray) and mean (black) nucleoporin intensity profiles measured on single z-slices are shown below for the indicated number of individual nuclei (n), 10 min before and 5 min after SPB separation. Scale bars, 3 μm.

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