Supporting data pertaining to nuclear basket behavior during S. pombe meiosis. (A) Maximum intensity projections (top row) and single z-slice image montages of cells with SpAlm1-GFP, a putative Tpr-like nucleoporin with no apparent S. cerevisiae ortholog (fySLJ842 × fySLJ479), progressing through meiosis I and staged according to the timing of meiosis I SPB separation as visualized using SpPpc89-mCherry (not shown). The individual (gray) and mean (black) SpAlm1-GFP intensity profiles measured on single z-slices are shown below for the indicated number of individual nuclei (n), 10 min before and after SPB separation. The white arrowhead in the “Fire LUT” images denote a nucleus after meiosis I SPB separation, at the stage when SpNup60-SpNup61 detachment is observed. (B–G) Montages of strains with the various GFP-tagged nucleoporins during meiosis II: (B) SpNup60-GFP (fySLJ730 × fySLJ479); (C) SpNup61-GFP (fySLJ840 × fySLJ479); (D) SpNup124-GFP (fySLJ456 × fySLJ989); (E) SpNup211-GFP (fySLJ456 × fySLJ990); (F) SpAlm1-GFP (fySLJ842 × fySLJ479); and (G) SpPom34-GFP (fySLJ1242 × fySLJ1243). Cells were staged according to the timing of meiosis II SPB separation as visualized using SpPpc89-mCherry. For B–G, the white arrowheads in the “Fire LUT” images denote nuclei after meiosis II SPB separation, at the time point when the second SpNup60-SpNup61 detachment occurs. Scale bars, 3 μm.