Meiosis I NPC remodeling is conserved in S. pombe. (A) A schematic depicting nuclear basket nucleoporin orthologs in S. cerevisiae and S. pombe. (B–F) Maximum intensity projections (top row) and single z-slice image montages of cells with various GFP-tagged nucleoporins progressing through meiosis I and staged according to the timing of meiosis I SPB separation as visualized using SpPpc89-mCherry (not shown). For each nucleoporin, individual (gray) and mean (black) Nup-GFP intensity profiles measured on single z-slices are shown below for the indicated number of individual nuclei (n), 10 min before and after SPB separation. The following nucleoporins were visualized: (B) SpNup60-GFP, the ortholog of ScNup60 (fySLJ730 × fySLJ479); (C) SpNup61-GFP, the ortholog of ScNup2 (fySLJ840 × fySLJ479); (D) SpNup124-GFP, the ortholog of ScNup1 (fySLJ456 × fySLJ989); (E) SpNup211-GFP, the ortholog of ScMlp1 and ScMlp2 (fySLJ456 × fySLJ990); and (F) SpPom34-GFP, the ortholog of ScPom34 (fySLJ1242 × fySLJ1243). For B–F, the white arrowheads in the “Fire LUT” images denote nuclei after meiosis I SPB separation, at the stage when SpNup60-SpNup61 detachment is observed. Scale bars, 3 μm.