Supporting data pertaining to the role of Nup60 in gamete nuclear basket organization. (A and B) Montages of cells with Nup2-GFP, a nuclear basket nucleoporin, and Htb1-mCherry, a histone, progressing through meiosis in either (A) a NUP60 (UB15305) or (B) a nup60-ΔAH (UB30630) genetic background. (C and D) Montages of cells with Mlp1-GFP, a nuclear basket nucleoporin, and Htb1-mCherry, a histone, progressing through meiosis in either (C) a NUP60 (UB14648) or (D) a nup60-I36R (UB30640) genetic background. (E) Quantification of Mlp1-GFP organization for the strains in C and D during prophase I (defined as an hour before the post-MI time point), post-MI (defined as the presence of two clear Htb1-mCherry lobes), and post-MII (defined as 2 h after the presence of four clear Htb1-mCherry lobes). Sample sizes (n) indicate the number of nuclei scored for Mlp1 organization. For A–D, the white arrowheads in the “Fire LUT” images denote cells after anaphase II when gamete nuclear basket organization or misorganization is apparent. (F and G) Montages of cells with Nup170-GFP, a core nucleoporin, and Htb1-mCherry, a histone, progressing through meiosis in either (F) a NUP60 (UB11513) or (G) a nup60-ΔAH (UB34088) genetic background. (H and I) Montages of cells with Pom34-GFP, a core nucleoporin, and Htb1-mCherry, a histone, progressing through meiosis in either (H) a NUP60 (UB13503) or (I) a nup60-ΔAH (UB34086) genetic background. For all panels, the onset of anaphase II was defined by the presence of four Htb1-mCherry lobes. Scale bars, 2 μm.