Peripheral Nup60 is required for proper gamete nuclear basket organization. (A and B) Montages of cells with Nup1-GFP, a nuclear basket nucleoporin, and Htb1-mCherry, a histone, progressing through meiosis in either (A) a NUP60 (UB15303) or (B) a nup60-ΔAH (UB30628) genetic background. (C) Quantification of average nuclear envelope intensity of Nup1-GFP during prophase I (defined as 1 h prior to the anaphase I, the presence of two clear Htb1-mCherry lobes) in NUP60 and nup60-ΔAH cells, as shown in A and B. Individual intensity values were normalized to the average nuclear envelope intensity in NUP60 cells. Asterisks indicate significance determined using a Wilcoxon rank sum test (see Table S5 for P values). Sample sizes (n) are the number of cells quantified in each background. (D and E) Montages of cells with Mlp1-GFP, a nuclear basket nucleoporin, and Htb1-mCherry, a histone, progressing through meiosis in either (D) a NUP60 (UB14648) or (E) a nup60-ΔAH (UB30632) genetic background. (F) Quantification of Mlp1-GFP organization for the strains in D and E during prophase I (defined as an hour before the post-MI time point), post-MI (defined as the presence of two clear Htb1-mCherry lobes), and post-MII (defined as 2 h after the presence of four clear Htb1-mCherry lobes). Sample sizes (n) indicate the number of nuclei scored for Mlp1 organization. For panels A, B, D, and E, the white arrowheads in the “Fire LUT” images denote cells after anaphase II when gamete nuclear basket organization or misorganization is apparent. For all panels, the onset of anaphase II was defined by the presence of four Htb1-mCherry lobes. Scale bars, 2 μm.