Cdc5-dependent Nup60 phosphorylation mediates meiosis I, but not meiosis II, NPC remodeling. (A and B) Montages of cells with (A) Nup60-GFP (UB14646) or (B) Nup60^9A-GFP (UB29358) and Htb1-mCherry progressing through meiosis. (C) Quantification of Nup60 detachment before (−10 min, “Pre”), coincident with (0 min, “Anaphase I”), and after (+10 min, “Post”) the onset of anaphase I. Individual DI values were normalized to the average DI of Nup60-GFP at the “Pre” time point. Asterisks indicate statistical significance calculated using Dunn’s test for multiple comparisons when each allele was compared to Nup60-GFP for a given time point (see Table S5 for P values). The dashed lines indicate the average DI for Nup60-GFP for each time point. Sample sizes (n) are the number of cells quantified for each allele; for Nup60-GFP, cells from two independent replicates were pooled. (D) A schematic that depicts Cdc5-dependent phosphorylation of Nup60 driving detachment from the NPC during meiosis I in wild type, but not phosphomutant, cells. For all panels, the onset of anaphase I was defined as the Htb1-mCherry chromatin mass exhibiting distortion from a spherical shape consistent with chromosome segregation. For each montage, normalized DI values are indicated when calculated. Scale bars, 2 μm.