Two distinct NPC remodeling events occur during budding yeast meiosis. (A) SIM images of fixed cells with Nup2-GFP, a nuclear basket nucleoporin, and Pom34-mCherry, a transmembrane nucleoporin (UB20080). (B) SIM of fixed cells with Nup84-GFP, a Y-complex nucleoporin, and Pom34-mCherry, a transmembrane nucleoporin (UB21079). For A and B, the white arrowheads in the “Merge” images denote the GUNC. (C) Schematic of the FKBP12-FRB inducible dimerization approach used to tether Nup60, a nuclear basket nucleoporin, to Seh1, a Y-complex nucleoporin. (D and E) Montages of cells containing FKBP12-Nup60-GFP and Seh1-FRB, treated with either (D) DMSO or (E) 10 μM rapamycin after 4 h in SPM (UB27298). (F) Montages of cells with different fluorescently tagged basket nucleoporins—Nup2-GFP (UB25843), Nup1-GFP (UB27143), and Mlp1-GFP (UB27725)—and the inducible Nup60 tether (FKBP12-Nup60 and Seh1-FRB) treated with 10 μM rapamycin after 4 h in SPM. For D–F, the transmembrane nucleoporin Pom34-mCherry was used to monitor the NPC core, with the GUNC indicated by a white box. The onset of anaphase II was defined as the first time point with GUNC formation. All cells were fpr1Δ to facilitate rapamycin access to the tether. (G) Model depicting the two distinct NPC remodeling events that occur during budding yeast meiosis: (1) partial basket detachment (Nup60 and Nup2) during meiosis I and (2) full basket detachment (Nup60, Nup2, Nup1, and Mlp1) during meiosis II. Note that, although Nup1 is depicted as remaining associated with the NPC core during meiosis I, it exhibits moderate detachment. Mlp2 is not shown as we were unable to monitor its localization during meiosis. Scale bars, 2 μm.