Figure S2. Supporting data pertaining to... | Rockefeller University Press

$Supporting data pertaining to nuclear basket behavior during S. cerevisiae mitosis. (A–E) Montages of strains with GFP-tagged nucleoporins and a fluorescently tagged histone Htb1-mCherry during mitosis. (A) Nup60-GFP (UB14646); (B) Nup2-GFP (UB15305); (C) Nup1-GFP (UB15303); (D) Mlp1-GFP (UB14648); and (E) Pom34-GFP (UB13503). Mitotic anaphase was defined as the first time point when a significant fraction of the histone mass had traversed the bud neck; the white arrowheads in the “Fire LUT” images denote nuclei at this stage. (F) Quantification of nucleoporin detachment before (−15 min for mitosis and −10 min for meiosis; “Pre”) and coincident with mitotic anaphase or meiotic anaphase I (0 min; “Anaphase”). The detachment index (DI) for individual cells was calculated from single z-slices using a line profile method. For each nucleoporin during mitosis or meiosis, individual DI values were normalized to the average DI at the “Pre” time point. Asterisks indicate statistical significance calculated using Dunn’s test for multiple comparisons when each nucleoporin was compared to Pom34-GFP, a transmembrane nucleoporin, for a given time point and type of cell division (see Table S5 for P values). Sample sizes (n) are the number of cells quantified for each nucleoporin. Scale bars, 2 μm.$

Figure S2.

Supporting data pertaining to nuclear basket behavior during S. cerevisiae mitosis. (A–E) Montages of strains with GFP-tagged nucleoporins and a fluorescently tagged histone Htb1-mCherry during mitosis. (A) Nup60-GFP (UB14646); (B) Nup2-GFP (UB15305); (C) Nup1-GFP (UB15303); (D) Mlp1-GFP (UB14648); and (E) Pom34-GFP (UB13503). Mitotic anaphase was defined as the first time point when a significant fraction of the histone mass had traversed the bud neck; the white arrowheads in the “Fire LUT” images denote nuclei at this stage. (F) Quantification of nucleoporin detachment before (−15 min for mitosis and −10 min for meiosis; “Pre”) and coincident with mitotic anaphase or meiotic anaphase I (0 min; “Anaphase”). The detachment index (DI) for individual cells was calculated from single z-slices using a line profile method. For each nucleoporin during mitosis or meiosis, individual DI values were normalized to the average DI at the “Pre” time point. Asterisks indicate statistical significance calculated using Dunn’s test for multiple comparisons when each nucleoporin was compared to Pom34-GFP, a transmembrane nucleoporin, for a given time point and type of cell division (see Table S5 for P values). Sample sizes (n) are the number of cells quantified for each nucleoporin. Scale bars, 2 μm.

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