Supporting data pertaining to the meiotic behavior of nucleoporins from various NPC subcomplexes. (A) Quantification of Nup60-GFP detachment and reattachment timing relative to anaphase I onset, corresponding to Fig. 1 D. The mean ± range (shaded range) of two independent biological replicates is displayed (n = 58 cells for replicate 1 and 53 cells for replicate 2). (B–F) Montages of cells with different fluorescently tagged nucleoporins and Htb1-mCherry, a histone, progressing through meiosis: (B) Nup2-GFP, a nuclear basket nucleoporin (UB15305); (C) Nup1-GFP, a nuclear basket nucleoporin (UB15303); (D) Nup49-GFP, a channel nucleoporin (UB13509); (E) Nup120-GFP, a Y-complex nucleoporin (UB13499); and (F) Nup170-GFP, an inner ring complex nucleoporin (UB11513). For each montage, normalized DI values (relative to the average value at the pre-anaphase I time point for each nucleoporin) are indicated when calculated. The onset of anaphase I was defined as the Htb1-mCherry chromatin mass exhibiting distortion from a spherical shape consistent with chromosome segregation. The white arrowheads in the “Fire LUT” images denote nuclei at the onset of anaphase I, the stage when Nup60-Nup2 detachment is observed. Scale bars, 2 μm.