Direct stimulation of ASIC1 causes vasodilation and activates IK _Ca /SK _Ca currents. (A and B) Representative trace showing changes in vessel inner diameter (µm; A) and summary data showing percent vasodilation in response to luminal perfusion of α/β-MitTx (200 nM bolus; B) in preconstricted mesenteric arteries in the presence of vehicle, PcTX1, LNNA-Indo, or LNNA-Indo-TRAM/Ap. Data represent biological replicates and were analyzed by one-way ANOVA. Post-hoc pairwise comparisons were analyzed between individual groups using Tukey’s multiple comparisons test. (C and D) Representative K⁺ current traces (C) and average current density (pA/pF; D) plotted as a function of voltage (mV) recorded in freshly dispersed mesenteric endothelial cells during baseline (from Fig. 7 B) and following activation of ASIC1 with α/β-MitTx (20 nM; purple diamond) in the absence or presence of PcTX1 (20 nM, red squares) or Tram-34 (1 µM) and Apamin (100 nM; blue triangles). Data represent biological replicates (noted in parentheses) and were analyzed by two-way ANOVA. The two-way ANOVA revealed that there was a statistically significant interaction (P < 0.0001) between factors. (E and F) Post-hoc pairwise comparisons were analyzed between individual groups using Tukey’s multiple comparisons test and the P values are given in Table 3 and in summary data for −140 mV (E) and 100 mV (F).