ASIC1 inhibition abolishes ACh-induced IK _Ca /SK _Ca currents. (A) Representative fluorescence and brightfield images showing identification of lectin-stain endothelial cells. (B–D) Representative K⁺ current traces and average current density (pA/pF) plotted as a function of voltage (mV) recorded in freshly dispersed mesenteric endothelial cells during baseline conditions (C) and following 1 µM ACh (D) in the presence of vehicle (black circles), PcTX1 (20 nM, red triangles), or Tram-34 (1 µM) and Apamin (100 nM; blue squares). Data represent biological replicates (noted in parentheses) and were analyzed by two-way ANOVA. The two-way ANOVA revealed that there was a statistically significant interaction (P < 0.0001) between factors. (E and F) Post-hoc pairwise comparisons were analyzed between individual groups using Tukey’s multiple comparisons test and the P values are given in Table 3 and summary data for −140 mV (E) and 100 mV (F). (G) Summary data showing Tram/Ap-sensitive and PcTX1-sensitive K⁺ currents under baseline conditions or following ACh.