Augmented basal tone and blunted ACh-induced vasodilation in mesenteric arteries from Asic1a ^−/− mice. (A and B) Basal tone (percent passive diameter; A) and basal vessel wall intracellular [Ca²⁺] (F₃₄₀/F₃₈₀; B) in mesenteric arteries from Asic1a^+/+ or Asic1a^−/− mice. (C and D) Vasoconstriction (percent baseline inner diameter; C) and changes in the vessel wall [Ca²⁺] (Δ F₃₄₀/F₃₈₀; D) in response to Ang II (10⁻⁷ M). (E) Averaged traces showing changes in vessel inner diameter (µm) in response to ACh (10⁻⁶ M) in preconstricted mesenteric arteries from Asic1a^+/+ or Asic1a^−/− mice. (F and G) Summary data showing percent peak (F) and sustained (G) vasodilatory responses to ACh. Biological replicates were analyzed by unpaired t tests.