![ASIC1a contributes to ACh-induced vasodilation in mesenteric arteries. (A) Basal vessel wall intracellular [Ca2+] (F340/F380) in mesenteric arteries in the presence of vehicle or PcTX1 (20 nM). (B and C) Vasoconstriction (percent baseline inner diameter; B) and changes in vessel wall [Ca2+] (Δ F340/F380) in response to PE (10−8 to 10−5 M; C) in the presence of vehicle or PcTX1. (D) Representative traces showing changes in vessel inner diameter (µm) in response to increasing doses of ACh (10−9 to 10−5 M) in preconstricted mesenteric arteries. (E and F) The percent peak (E) and sustained (F) vasodilatory responses to ACh in the presence or absence of PcTX1. n = 5–6 animals (biological replicates)/group, analyzed by unpaired t tests, *P < 0.05 vs. vehicle. P values for data in E from 10−9 to 10−5 M ACh are 0.0158, 0.0384, 0.0017, 0.1907, 0.2096, 0.9620, 0.1264, 0.0415, and 0.0430. P values for data in F from 10−9 to 10−5 M ACh are 0.0269, 0.0661, 0.0031, 0.1524, 0.0884, 0.0151, 0.0008, 0.0014, and 0.0041.](https://cdn.rupress.org/rup/content_public/journal/jgp/155/2/10.1085_jgp.202213173/1/jgp_202213173_fig2.png?Expires=1767742578&Signature=zkY5pFT~v526Hquse1pHSL5jHEXostWIcAhFcSV7KRDAdyIac1Xi0EZLoVWm8f9LPz824RNlNJHUvFupDI~JkyAhcGCe4tUWZhN6Rc7SOJqFrhPInNLhv5JUGhY9EM-r~p8MjFrCJYhcaGFh6ddU2cll2Wq57hXthwBA~DGGDwY3SBDriQCCSB3PWpLp5ypaRXxbXfaomhbx8UgYR2W2OajhZ2AOuWz~2xY0bt2E15OdHwgvQLOEZ34InBN2Tq~UigGlnM7OduGSisS8ru~kkpATWn71ojSEB22mC3BYbACu1CaexeEU9rT8yo~7lnedfBkw5jWRs0TpvPYcCHhA7A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
ASIC1a contributes to ACh-induced vasodilation in mesenteric arteries. (A) Basal vessel wall intracellular [Ca2+] (F340/F380) in mesenteric arteries in the presence of vehicle or PcTX1 (20 nM). (B and C) Vasoconstriction (percent baseline inner diameter; B) and changes in vessel wall [Ca2+] (Δ F340/F380) in response to PE (10−8 to 10−5 M; C) in the presence of vehicle or PcTX1. (D) Representative traces showing changes in vessel inner diameter (µm) in response to increasing doses of ACh (10−9 to 10−5 M) in preconstricted mesenteric arteries. (E and F) The percent peak (E) and sustained (F) vasodilatory responses to ACh in the presence or absence of PcTX1. n = 5–6 animals (biological replicates)/group, analyzed by unpaired t tests, *P < 0.05 vs. vehicle. P values for data in E from 10−9 to 10−5 M ACh are 0.0158, 0.0384, 0.0017, 0.1907, 0.2096, 0.9620, 0.1264, 0.0415, and 0.0430. P values for data in F from 10−9 to 10−5 M ACh are 0.0269, 0.0661, 0.0031, 0.1524, 0.0884, 0.0151, 0.0008, 0.0014, and 0.0041.