Wld ^S overexpression in neurons rescues neurodegeneration in babo knockdown animals. (A) Representative images of neuron cell bodies and axons within the wing in control and babo knockdown animals at 4 (top) and 28 (bottom) days of age with and without Wld^S expressed in neurons. Scale bar 5 μm. (B) Classification of axon integrity in control and babo knockdown animals at 28 d with and without Wld^S. Pairwise Fisher Exact Probability test (two-tailed, 2 × 3, n = 17 [control], 19 [control +Wld^S], 27 [babo^RNAi], 20 [babo^RNAi +Wld^S]). (C) Quantification of the number of VGlut⁺ neuron cell bodies in the wing from control and babo knockdown animals with and without Wld^S (n = 40 [control], 46 [control +Wld^S], 53 [babo^RNAi], 48 [babo^RNAi +Wld^S]). Mean ± 95% CI. Two-way ANOVA with Tukey’s multiple comparisons test, n = 187. *, P < 0.05; ****, P < 0.0001; ns, not significant.