aPKC accumulates at the posterior after the changes in Bazooka and Par-1. (A) Two-color time-lapse images of an egg chamber at stage 9, expressing endogenous aPKC tagged with EGFP (green) and Baz::mCherry in the germline (magenta) before (−1 min) and after ablation of PFCs (yellow dashed circle). Scale bar, 20 µm (B) Zoom-in of the posterior of the egg chamber shown in A. Top: Baz::mCherry, bottom: EGFP::aPKC. While Bazooka accumulates within 60 min, aPKC is detectable only after 120–150 min (arrowheads). Scale bar, 20 µm. (C) Baz::mCherry intensity at the oocyte cortex facing the ablated PFCs, as a function of time for oocytes at stage 9 (orange, n = 6) and 10A (blue, n = 3). Thin lines are individual experiments, thick lines are stage averages. (D) As in C but for EGFP::aPKC. Note the delayed increase in stage 9, and the lack of signal increase in stage 10A. (E) Normalized average intensities of GFP::Par-1 (dashed line) and EGFP::aPKC (solid line) at stage 9 as a function of time following the ablation of PFCs. Note the delay of aPKC signal increase. For all panels, time zero marks the first frame after ablation.