Activating NO–sGC signaling restores EC–pericyte crosstalk. (A) Heatmap showing the LPS-dysregulated EC–pericyte interaction pairs that were reversed by Riociguat treatment. Doughnut charts on the right showing the percentage of Riociguat-reversed interactions among the total LPS-dysregulated interaction pairs. (B) Plot showing the LPS-dysregulated interactions Nos3-Gucy1a1 and Nos3-Gucy1b1 were rescued by Riociguat treatment. Data are presented as mean ± SD, n = 5 mice. (C) The protein levels of eNOS and GUCY1A1 were validated by Western blot analysis. (D) Plots shows the quantification of eNOS, GUCY1A1, and GUCY1B1 normalized to TUBULIN. Data are presented as mean ± SD, n = 4–5 mice. Statistical significance was determined by one-way ANOVA with Tukey test. Source data are available for this figure: SourceData F9.