Administration of anti–TLT-1 antibody substantially reduces tumor growth in mice. (A) Quantitation of soluble TLT-1 in mouse plasma as measured by ELISA in all four study groups. (B and C) Representative mouse tumor immunofluorescence confocal images (n = 5 biological samples with at least 10 micrographs captured per section). The scale bar equals 50 µm. The mouse tumor tissue invasive margins showing CD8 (column first from left) and CD4 (second from left) in four study groups with significant infiltration of CD8 TILs in tumor (Veh) + anti–TLT-1 antibody group, and the quantitation (C) of mean intensity from samples of each group quantified together for intergroup comparisons. The scatter plots show mean ± SEM analysed by Kruskal–Wallis test with Dunn’s multiple comparisons. Significant differences are indicated with asterisks (*, P < 0.05; **, P < 0.01). The data represent the results of two independent experiments.