Identification of epigenetic marker regions in LN-derived ILCs. (A) Heatmaps showing the methylation level of selected epigenetic marker regions for ILC1, ILC2, ILC3, and LTi cells. Short DMRs produced by metilene software were extended to include adjacent differentially methylated CpG motifs to generate marker regions. The regions were named after the associated gene locus and numbered if more than one region was linked to a locus. The mean methylation value was calculated from the CpG motifs located within the marker region. The values were translated into a color code ranging from yellow (0% methylation = 0) via white (50% methylation = 0.5) to blue (100% methylation = 1.0). (B) Methylation profiles of marker-associated gene loci. Smoothed, linear display of CpG motifs (bar code), methylation values of the DMR (light gray box), and the surrounding gene body (exons in dark gray boxes, TSS indicated by arrow). Colored lines depict the methylation values ranging from 0 (0% methylation) to 1 (100% methylation) for each ILC subset (blue, ILC1; red, ILC2; cyan, ILC3; dark magenta, LTi; black, NK). Three selected gene loci for ILC1/NK (top), ILC2 (middle), and ILC3/LTi (bottom) are shown.