Genome-wide methylation analysis of LN-derived ILCs. For initial WGBS, ILC populations were pooled from >10 independent sorts of a total of n = 200 mice. WGBS was performed in unicates for NK cells, ILC1, ILC2, ILC3, and LTi cells. (A) Number of DMRs among ILC populations in pairwise comparisons. Methylomes were built from bisulfite sequencing data that were mapped against the reference genome. Numbers indicated the DMRs discovered by metilene software, containing at least three CpG motifs and a 25% methylation difference. (B) Number of discovered DMRs at various distances (x axis) relative to the TSS of the closest gene. (C) Pie charts indicating the location of the DMRs identified in groupwise comparisons. Numbers show the frequency of DMRs in intergenic, intragenic, or promoter regions according to their genomic position. (D) Euclidian sample distances of DMR methylation values from pairwise comparisons. Distance value and associated color code (red to blue) is shown. (E) Identification of pathways that are associated with identified DMRs by a KEGG-based pathway enrichment analysis. The odds ratio of the resulting pathways from the indicated pairwise comparisons were translated into a color code and ordered according to their value.