scRNA-seq of WT and TGF-βRII–deficient P14 cells from chronically infected mice. (A) VISION analyses of WT and KO P14 cells for enrichment of gene signatures associated with LCMV-specific stem-like, transitory, and exhausted CD8 T cells. UMAP plots identified the cells enriched for each gene signature shown in blue color. Violin plots showed the enrichment of each gene signature in the clusters of WT and KO P14 cells (stem, transitory, and exhausted). (B) Dot plots showing the relative expression levels and the percentages of cells in each cluster expressing the selected genes.