Dysregulated phosphosites and protein levels in brain capillaries of E4F compared with E3F mice. (A) Pie chart showing distribution of phosphosites with either increased or decreased levels of phosphorylation in brain capillaries from 7-mo-old E4F compared with E3F mice. (B) GO enrichment analysis of all nonredundant proteins with differentially regulated phosphosites. Enrichment is classified by terms indicating molecular function (red), cellular component (orange), and biological process (blue). (C) Pie chart showing distribution of predicted kinase family-substrate pairs for all dysregulated phosphosites in brain capillaries from 7-mo-old E4F compared with E3F mice. (D) Distribution of predicted kinase family-substrate pairs for all dysregulated phosphosites by subcellular location. Abbreviations for protein kinase families in C and D are the same as in main Fig. 3, H and K. (E and F) GO enrichment of all nonredundant proteins regulated by phosphorylation and assigned to specific cellular components of the BBB, including ECs and PCs. Enrichment is classified by terms indicating molecular function, cellular component, and biological process as in B. (G) Venn diagram showing the number of proteins overlapping between proteins found to contain differentially regulated phosphosites and proteins found to be differentially expressed in brain capillaries from 7-mo-old E4F compared with E3F mice. (H) Pie chart showing distribution of proteins found to have either increased or decreased levels in E4F compared with E3F mice. (I) Graphs showing functional categories of differentially expressed proteins separated by direction of regulation and assigned cell type as ECs or PCs. All data in are from four mice per group. All reported P values are adjusted using the Bonferroni correction for multiple comparisons.