Cellular composition of isolated mouse brain capillaries. (A–D) Isolated brain capillaries stained for lectin⁺-endothelium (A; lectin, green; DRAQ5 nuclear stain, pink; bar, 100 µm), Pdgfrβ⁺-PCs (B; Pdgfrβ, red; lectin⁺-endothelium, green; DRAQ5, blue; bar, 10 µm), and AQP4⁺-astrocyte end feet (C, AQP4, green, lectin⁺-endothelium, red; DRAQ5, blue; bar, 50 µm) and did not stain for smooth muscle cell marker SMA (D, lectin, green; SMA, red; bar, 50 µm).