IFN-γ blockade delays spontaneous intestinal inflammation in BATF-deficient cKO mice. (A) Experiment design showing the timeline for co-housed cKO mice intraperitoneally injected with anti–IFN-γ (a-IFNγ) or isotype-IgG control antibody, started at week 4. (B) Mice injection with anti–IFN-γ or isotype-IgG control antibody were monitored for incidence of rectal prolapse. (C) H&E staining of ileum and colon sections from mice injection with anti–IFN-γ or isotype-IgG control antibody at week 14. Scale bar, 100 µm. (D) Quantification of the histological score of ileum and colon as assessed in C. (E) qRT-PCR analysis of relative abundance of fecal microbiome from mice injection with anti–IFN-γ or isotype-IgG control antibody at week 8 and 14. Statistical differences were tested using two-way ANOVA for B, unpaired t test for D, and one-way ANOVA for E, n = 5–8 mice per group. Data are pooled from two independent experiments. *P < 0.05, **P < 0.01.