BATF regulates SI ILC3s transcriptional program. Related to Fig. 2. (A) Heatmap of wound healing signature genes from WT versus cKO SI ILC3s. (B–D) GSEA analysis of pathways differentially enriched in WT or cKO SI ILC3s. Normalized enrichment score (NES) and adjusted P value are indicated for each gene set. (B) GSEA plot of the positive regulation of wound-healing pathway. (C) GSEA dot plot of select GO pathways enriched in WT and cKO SI ILC3s. The size and color of each dot indicate the number of genes in a pathway and the adjusted P value, respectively. (D) GSEA plot of the IBD pathway. (E) Flow cytometry analysis subsets of intestinal ILC3 (Lin⁻CD90.2⁺Rorγt⁺) of cKO and littermate control WT mice aged 13 wk. (F) Flow cytometry histograms analyzing the expression of BATF in three subsets of ILC3 as assessed in E. (G) Quantification of MFI of BATF on ILC3s subsets as assessed in E. (H) The expression of indicated genes from cKO versus WT SI ILC3s via RNA-seq analysis. (I) Genomic snapshots showing accessibility (ATAC-seq) of genes as indicated in H in Rag1^−/− and Rag1^−/−Batf^−/− ILC3s. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed unpaired t test). Each dot represents one mouse, n = 4–5 mice per group. Data are representative of at least two independent experiments.