BATF deficiency in ILCs alters the heterogenicity of SI ILCs. (A) Flow cytometry gating strategy and analysis of different ILC subsets in the SI of WT and cKO mice. (B–D) Cell frequency and numbers of ILC1s (Lin⁻CD90.2⁺T-bet⁺Rorγt⁻; B), ILC2s (Lin⁻CD90.2⁺T-bet⁻Rorγt⁻GATA3⁺; C) and ILC3s (Lin⁻CD90.2⁺Rorγt⁺; D) in the SI of WT and cKO mice. (E) Cell numbers of ILCs in various tissues of WT and cKO mice. (F) Flow cytometry analysis of the frequency of MHCII⁺ ILC3 in the colon of cKO and littermate control WT mice aged 13 wk. (G) Quantified frequencies of MHCII⁺ ILC3 in the colon as assessed in (F). (H) Quantification of MFI of MHCII on ILC3 in the colon as assessed in (F). Data are shown as mean ± SEM. Statistical differences were tested using a paired t test (two-tailed; B and C) or two-way ANOVA for D. **P < 0.01. Each dot represents one mouse, n = 6 mice per group. Data are pooled from two independent experiments.