Western blot analyses of proteins that coordinate SOCE in EDL muscle. (A and B) Representative immunoblots showing expression levels of the long (STIM1L) and short (STIM1S) STIM1 splice variants (A) and Orai1 (B) in EDL muscle homogenates from WT (n = 6, C; n = 4, D), CASQ1-null (n = 6, C; n = 4, D), and dCASQ-null (n = 6, C; n = 4, D) mice. For Orai1 expression, EDL muscles from constitutive, muscle-specific Orai1 KO (cOrai1 KO) mice (n = 2) were used as a negative control to validate the Orai1 antibody used. (C and D) Relative band intensities (normalized to GAPDH) for each of the proteins shown in A and B. Data are shown as mean ± SEM. Statistical significance: C: *, P = 0.0095 and *, P = 0.002 for STIM1L and STIM1S, respectively, WT vs. CASQ1-null; *, P = 0.04 and *, P = 0.0016 for STIM1L and STIM1S, respectively, WT vs. dCASQ-null; D: *, P = 0.00093, WT vs. CASQ1-null; *, P = 0.00028, WT vs. dCASQ-null. Source data are available for this figure: SourceData FS3.