Geometric features are robust to changes in microscope resolution. (a) PCA comparison between original data and simulations of threefold blurred data in Z-dimension causing non-isotropic resolution. The two datasets are largely overlapping, demonstrating minimal effect of decreased axial resolution on shape feature measurements. (b) Comparison of XY (top) and XZ (middle) projections and 3D rendering (bottom) of the same cell acquired either on the ASLM (high axial resolution) or mSPIM (low axial resolution) show similarities despite decreased resolution. (c) Comparison of all 12 shape features (V, volume; SA, surface area; LL, long length; VS, volume sphericity; Sph, sphericity; AR, aspect ratio; Sol, solidity; R, roughness; Ext, extent; CSR, circumscribed surface ratio; RdS, radius sphericity; RtS, ratio sphericity) in four cells imaged in both mSPIM and ASLM show no significant difference (ns, paired t test). (d) Overall shift in PC space of the same cell imaged in ASLM (white) or mSPIM (black) is modest. Scale bar, 10 µm.