Figure 3. Absence of subsynaptic IP 3... | Rockefeller University Press

Figure 3.

Absence of subsynaptic IP3R1s at endplate level in 9-d denervated rat EDL longitudinal sections. (A) In the left panel, contralateral EDL labeled with anti-IP3R1 antibodies; in the right panel, merged image after double labeling for nAChRs and anti-IP3R1s. Scale bar, 20 μm. (B) Individual labeling of contralateral EDL with α-BuTX, anti-IP3R1 antibodies, and DAPI. Scale bar, 20 μm. (C) Individual labeling of 9-d denervated EDL with α-BuTX, anti-IP3R antibodies, and DAPI. Scale bar, 20 μm.

Absence of subsynaptic IP ₃ R1s at endplate level in 9-d denervated rat EDL longitudinal sections. (A) In the left panel, contralateral EDL labeled with anti-IP₃R1 antibodies; in the right panel, merged image after double labeling for nAChRs and anti-IP₃R1s. Scale bar, 20 μm. (B) Individual labeling of contralateral EDL with α-BuTX, anti-IP₃R1 antibodies, and DAPI. Scale bar, 20 μm. (C) Individual labeling of 9-d denervated EDL with α-BuTX, anti-IP₃R antibodies, and DAPI. Scale bar, 20 μm.