No change in endplate and α-BuTX–stained volume was observed in 9-d denervated rat EDL longitudinal sections. (A) Representative images for contralateral and denervated endplates. Scale bar, 20 μm. (B) nAChR-stained volumes measured in confocal images from contralateral and denervated EDL are shown as a scatter plot (contralateral: 33 endplates; denervated: 39 endplates). White and grey boxes represent the mean volume ± SEM. ns, P = 0.06, Mann–Whitney test: data are not significantly different.