Analysis of mice treated with 4PBA. (A) Experimental design of 4PBA treatment during weeks 8 through 16 of control (C) sleep or SF. (B) Body weight, data of C and SF mice are as in Fig. S1 (n = 5–6 mice per group). (C) Proportion of BM LSKs (n = 3–5 mice per group). (D) Expression of Cdkn1b, Cdkn2b, Cdkal1, Klf3, and Klf9 genes in sorted LSKs; data from control and SF mice are as in Fig. 2 G (n = 4–7 mice per group).