Figure S4.
Erg6 expression in HeLa cells does not significantly vary between experiments. (A) HeLa cells transfected for 24 h with Erg6-eGFP and ER marker RFP-KDEL and then either untreated or incubated with 400 µM of OA or 250 µM of methyl-β-cyclodextrin conjugated CHOL for 24 h. Scale bars indicated. LDs stained with LipidTox. (B) HeLa cells transfected for 24 h with Erg6-DsRed2 and ER marker (ERox-BFP) and then either untreated or incubated with 400 µM of OA or 250 µM of methyl-β-cyclodextrin conjugated CHOL for 24 h. Scale bars indicated. LDs stained with LipidTox. (C) Quantification of Erg6 total fluorescence signal (a.u.) in cells from A or B. Cells used for total cell fluorescence quantification: n = 20 for Erg6-eGFP + OA, 21 for Erg6-eGFP + Chol, 19 for Erg6-DsRed2 + OA, 22 for Erg6-DsRed2 + Chol. All scale bars indicated.

Erg6 expression in HeLa cells does not significantly vary between experiments. (A) HeLa cells transfected for 24 h with Erg6-eGFP and ER marker RFP-KDEL and then either untreated or incubated with 400 µM of OA or 250 µM of methyl-β-cyclodextrin conjugated CHOL for 24 h. Scale bars indicated. LDs stained with LipidTox. (B) HeLa cells transfected for 24 h with Erg6-DsRed2 and ER marker (ERox-BFP) and then either untreated or incubated with 400 µM of OA or 250 µM of methyl-β-cyclodextrin conjugated CHOL for 24 h. Scale bars indicated. LDs stained with LipidTox. (C) Quantification of Erg6 total fluorescence signal (a.u.) in cells from A or B. Cells used for total cell fluorescence quantification: n = 20 for Erg6-eGFP + OA, 21 for Erg6-eGFP + Chol, 19 for Erg6-DsRed2 + OA, 22 for Erg6-DsRed2 + Chol. All scale bars indicated.

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