Figure 4. Electrically evoked Ca 2... | Rockefeller University Press

Electrically evoked Ca2+ transients in intact FDB fibers during prolonged exposure to BTP2.(A) Representative mag-fluo-4 traces before (0 min, left), after 10 min exposure (middle), and after 20 min exposure (right) to DMSO (black), 10 µM BTP2 (blue), or 20 µM BTP2 (red) during continuous electrical stimulation for 30 min at 0.1 Hz. (B) Average (± SEM) peak ΔF/F0 at 5-min intervals throughout 30 min exposure to DMSO (black bars), 10 µM BTP2, (blue bars), or 20 µM BTP2 (red bars). n = 13–26 cells from three mice. *, Significantly different from DMSO; P = 0.0069 for 10 min; P = 0.0012 for 15 min; P = 0.0004 for 20 min; P = 0.0009 for 25 min; P < 0.0001 for 30 min, 20 µM BTP2; and P = 0.0215 for 30 min, 10 µM BTP2. ^, Significantly different from 10 µM BTP2; P = 0.0031 for 10 min; P = 0.0025 for 15 min; P = 0.0026 for 20 min; and P = 0.0151 for 25 min.

Figure 4.

Electrically evoked Ca²⁺ transients in intact FDB fibers during prolonged exposure to BTP2. (A) Representative mag-fluo-4 traces before (0 min, left), after 10 min exposure (middle), and after 20 min exposure (right) to DMSO (black), 10 µM BTP2 (blue), or 20 µM BTP2 (red) during continuous electrical stimulation for 30 min at 0.1 Hz. (B) Average (± SEM) peak ΔF/F₀ at 5-min intervals throughout 30 min exposure to DMSO (black bars), 10 µM BTP2, (blue bars), or 20 µM BTP2 (red bars). n = 13–26 cells from three mice. *, Significantly different from DMSO; P = 0.0069 for 10 min; P = 0.0012 for 15 min; P = 0.0004 for 20 min; P = 0.0009 for 25 min; P < 0.0001 for 30 min, 20 µM BTP2; and P = 0.0215 for 30 min, 10 µM BTP2. ^, Significantly different from 10 µM BTP2; P = 0.0031 for 10 min; P = 0.0025 for 15 min; P = 0.0026 for 20 min; and P = 0.0151 for 25 min.

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