Superimposed traces of electrically evoked Ca²⁺ transients in intact FDB fibers on an expanded time scale. (A and B) Overlay of representative mag-fluo-4 traces with the rising phase on an expanded time scale for the fibers presented in the inset to Fig. 1 D before (0 min; A) and after (2 min; B) exposure to DMSO (black), 10 µM BTP2 (blue), or 20 µM BTP2 (red). Inset in A indicates the region of the traces (in orange box) presented on the expanded time scale in A–E. (C–E) Overlay of representative superimposed traces on an expanded time scale before and after 2 min exposure to DMSO (C), 10 µM BTP2 (D), or 20 µM BTP2 (E). (F and G) Overlay of the decay phase of mag-fluo-4 traces on an expanded time scale for the representative fibers presented in the inset to Fig. 1 D before (0 min; F) and after (2 min; G) exposure to DMSO (black), 10 µM BTP2 (blue), and 20 µM BTP2 (red). Inset in panel F indicates the region of the traces (in orange box) presented on the expanded time scale in F and G. Fluorescence (F) of the representative traces was normalized to the peak fluorescence (F_max) for each fiber.