Table 2.

Pharmacological properties of swelling-activated anion currents

ConstructIC50 (μM)Hill coefficient
+100 mV−100 mV+100 mV−100 mV
Chimera 
8C-8A(IL125) 2.1 2.3 2.15 2.26 
8C-8A(IL125) E6A 0.28 0.33 1.18 1.28 
P < 0.0001 P < 0.0001 P < 0.005 P < 0.01 
8C-8A(IL125) M48D 412.9 109.1 0.37 0.48 
P < 0.0001 P < 0.0001 P < 0.0001 P < 0.0001 
8C-8A(IL125) M48K 36.9 21.7 0.65 1.53 
P < 0.0001 P < 0.0001 P < 0.0001 ​ 
8C-8A(IL125) M48R 10.2 19.4 0.75 1.33 
P < 0.0001 P < 0.0001 P < 0.0001 ​ 
8C-8A(IL125) L105F 2.1 2.4 1.91 1.87 
8C-8A(IL125) L105R 0.8 0.8 1.87 2.11 
P < 0.0001 P < 0.0001 ​ ​ 
8C-8A(IL125) Q106A 3.1 3.5 2.28 2.48 
P < 0.0005 P < 0.0001 ​ ​ 
8C-8A(IL125) Y108A 1.2 1.2 1.28 1.17 
P < 0.005 P < 0.0005 P < 0.05 P < 0.01 
8C-8A(IL125) N112A 5.0 5.9 2.03 2.47 
P < 0.0001 P < 0.0001 ​ ​ 
8C-8A(IL125) Y126F 0.40 0.47 1.26 1.30 
P < 0.0001 P < 0.0001 P < 0.05 P < 0.05 
8C-8A(IL125) L136E N.D. N.D. N.D. N.D. 
8C-8A(IL125) L136K 0.40 0.53 1.08 1.28 
P < 0.0001 P < 0.0001 P < 0.001 P < 0.005 
Heteromer 
8A + 8C 4.5 4.1 1.71 1.69 
8A E6A + 8C E6A 0.53 0.65 1.26 1.25 
P < 0.0001 P < 0.0001 ​ ​ 
8A T48D + 8C M48D 301.3 124.9 0.68 0.59 
P < 0.0001 P < 0.0001 ​ P < 0.05 
8A + 8C L105R 3.3 2.59 1.58 1.49 
​ P < 0.005 ​ ​ 
8A Y124F + 8C Y126F 3.4 3.1 1.75 1.73 
​ P < 0.05 ​ ​ 
8A L134E + 8C L136E 1.9 2.2 1.24 1.34 
P < 0.0001 P < 0.0001 ​ ​ 
8A L134K + 8C L136K 1.3 1.4 1.17 1.21 
P < 0.0001 P < 0.0001 ​ ​ 

P values reflect comparisons of WT 8C-8A(IL125) or 8A + 8C with mutants.

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