Electrophysiological properties of swelling-activated anion currents
| Construct | Erev | Amplitude at +120 mV | Rectification | Inactivation at +120 mV | Time constant at +120 mV |
|---|---|---|---|---|---|
| mV | pA/pF | I+120 mV/I−120 mV | I2sec/IMax | ms | |
| Chimera | |||||
| 8C-8A(IL125) | 9.9 ± 0.4 (7) | 158.9± 42.2 (7) | 1.80 ± 0.18 (7) | 0.60 ± 0.04 (7) | 2,244 ± 417 (7) |
| 8C-8A(IL125) E6A | 11.9 ± 0.5 (6) | 36.8 ± 3.4 (6) | 1.12 ± 0.04 (6) | 0.16 ± 0.02 (6) | 520 ± 77 (6) |
| | | P < 0.005 | P < 0.0001 | P < 0.005 | |
| 8C-8A(IL125) M48D | 2.4 ± 0.6 (8) | 81.9 ± 26.5 (8) | 1.84 ± 0.11 (8) | 0.83 ± 0.03 (8) | 1,356 ± 257 (8) |
| P < 0.0001 | | | P < 0.005 | | |
| 8C-8A(IL125) M48K | 10.6 ± 0.4 (5) | 145.3 ± 36.6 (5) | 2.46 ± 0.10 (5) | 0.69 ± 0.06 (5) | 1,056 ± 90 (5) |
| | | P < 0.005 | | | |
| 8C-8A(IL125) M48R | 10.2 ± 0.5 (5) | 347.6 ± 81.0 (5) | 3.97 ± 0.15 (5) | 0.70 ± 0.08 (5) | 1,534 ± 423 (5) |
| | P < 0.005 | P < 0.0001 | | | |
| 8C-8A(IL125) L105F | 3.8 ± 0.5 (6) | 22.1 ± 2.3 (6) | 1.72 ± 0.17 (6) | 0.85 ± 0.03 (6) | 1,281 ± 292 (6) |
| P < 0.0001 | | | P < 0.005 | | |
| 8C-8A(IL125) L105R | 12.4 ± 0.7 (5) | 88.0 ± 37.9 (5) | 1.11 ± 0.10 (5) | 0.28 ± 0.04 (5) | 989 ± 120 (5) |
| P < 0.05 | | P < 0.005 | P < 0.0001 | P < 0.05 | |
| 8C-8A(IL125) Q106A | 10.4 ± 0.6 (5) | 333.0 ± 48.4 (5) | 2.19 ± 0.18 (5) | 0.72 ± 0.03 (5) | 1,928 ± 648 (5) |
| | P < 0.05 | | | | |
| 8C-8A(IL125) Y108A | 8.6 ± 0.6 (5) | 161.5 ± 33.5 (5) | 1.40 ± 0.07 (5) | 0.95 ± 0.01 (5) | 1,834 ± 226 (5) |
| | | | P < 0.0001 | | |
| 8C-8A(IL125) N112A | 9.2 ± 0.9 (5) | 4.3 ± 0.3 (5) | 1.66 ± 0.10 (5) | 0.84 ± 0.06 (5) | 706 ± 243 (5) |
| | P < 0.05 | | P < 0.0001 | P < 0.05 | |
| 8C-8A(IL125) Y126F | 9.3 ± 0.3 (5) | 19.2 ± 5.9 (5) | 0.76 ± 0.07 (5) | 0.27 ± 0.05 (5) | 665 ± 120 (5) |
| | | P < 0.0001 | P < 0.0001 | P < 0.01 | |
| 8C-8A(IL125) L136E | 6.3 ± 0.5 (5) | 80.2 ± 25.4 (5) | 2.36 ± 0.08 (5) | 0.65 ± 0.09 (5) | 2,634 ± 585 (5) |
| P < 0.0005 | | P < 0.05 | | | |
| 8C-8A(IL125) L136K | 9.5 ± 0.6 (5) | 196.3 ± 54.7 (5) | 1.55 ± 0.11 (5) | 0.18 ± 0.01 (5) | 512 ± 44 (5) |
| | | | P < 0.0001 | P < 0.005 | |
| Heteromer | |||||
| 8A + 8C | 10.8 ± 0.3 (6) | 138.3 ± 46.0 (6) | 2.41 ± 0.25 (6) | 0.53 ± 0.06 (6) | 1,698 ± 289 (6) |
| 8A E6A + 8C E6A | 11.9 ± 0.4 (5) | 96.2 ± 33.9 (5) | 3.69 ± 0.60 (5) | 0.56 ± 0.10 (5) | 1,200 ± 202 (5) |
| 8A T48D + 8C M48D | 10.1 ± 0.7 (5) | 249.7 ± 93.7 (5) | 7.28 ± 0.76 (5) | 0.65 ± 0.09 (5) | 1,661 ± 315 (5) |
| | | P < 0.0001 | | | |
| 8A + 8C L105R | 12.0 ± 0.5 (5) | 202.0 ± 82.7 (5) | 2.05 ± 0.15 (5) | 0.30 ± 0.03 (5) | 880 ± 223 (5) |
| 8A Y124F + 8C Y126F | 11.4 ± 0.1 (5) | 102.0 ± 20.9 (5) | 2.19 ± 0.08 (5) | 0.31 ± 0.04 (5) | 1,060 ± 130 (5) |
| 8A L134E + 8C L136E | 11.3 ± 0.4 (5) | 154.5 ± 55.6 (5) | 3.20 ± 0.18 (5) | 0.87 ± 0.12 (5) | 994 ± 298 (5) |
| | | | P < 0.05 | | |
| 8A L134K + 8C L136K | 11.6 ± 0.5 (5) | 119.0 ± 49.4 (5) | 2.76 ± 0.25 (5) | 0.36 ± 0.05 (5) | 1,126 ± 232 (5) |
| Construct | Erev | Amplitude at +120 mV | Rectification | Inactivation at +120 mV | Time constant at +120 mV |
|---|---|---|---|---|---|
| mV | pA/pF | I+120 mV/I−120 mV | I2sec/IMax | ms | |
| 8C-8A(IL125) | 9.9 ± 0.4 (7) | 158.9± 42.2 (7) | 1.80 ± 0.18 (7) | 0.60 ± 0.04 (7) | 2,244 ± 417 (7) |
| 8C-8A(IL125) E6A | 11.9 ± 0.5 (6) | 36.8 ± 3.4 (6) | 1.12 ± 0.04 (6) | 0.16 ± 0.02 (6) | 520 ± 77 (6) |
| | | P < 0.005 | P < 0.0001 | P < 0.005 | |
| 8C-8A(IL125) M48D | 2.4 ± 0.6 (8) | 81.9 ± 26.5 (8) | 1.84 ± 0.11 (8) | 0.83 ± 0.03 (8) | 1,356 ± 257 (8) |
| P < 0.0001 | | | P < 0.005 | | |
| 8C-8A(IL125) M48K | 10.6 ± 0.4 (5) | 145.3 ± 36.6 (5) | 2.46 ± 0.10 (5) | 0.69 ± 0.06 (5) | 1,056 ± 90 (5) |
| | | P < 0.005 | | | |
| 8C-8A(IL125) M48R | 10.2 ± 0.5 (5) | 347.6 ± 81.0 (5) | 3.97 ± 0.15 (5) | 0.70 ± 0.08 (5) | 1,534 ± 423 (5) |
| | P < 0.005 | P < 0.0001 | | | |
| 8C-8A(IL125) L105F | 3.8 ± 0.5 (6) | 22.1 ± 2.3 (6) | 1.72 ± 0.17 (6) | 0.85 ± 0.03 (6) | 1,281 ± 292 (6) |
| P < 0.0001 | | | P < 0.005 | | |
| 8C-8A(IL125) L105R | 12.4 ± 0.7 (5) | 88.0 ± 37.9 (5) | 1.11 ± 0.10 (5) | 0.28 ± 0.04 (5) | 989 ± 120 (5) |
| P < 0.05 | | P < 0.005 | P < 0.0001 | P < 0.05 | |
| 8C-8A(IL125) Q106A | 10.4 ± 0.6 (5) | 333.0 ± 48.4 (5) | 2.19 ± 0.18 (5) | 0.72 ± 0.03 (5) | 1,928 ± 648 (5) |
| | P < 0.05 | | | | |
| 8C-8A(IL125) Y108A | 8.6 ± 0.6 (5) | 161.5 ± 33.5 (5) | 1.40 ± 0.07 (5) | 0.95 ± 0.01 (5) | 1,834 ± 226 (5) |
| | | | P < 0.0001 | | |
| 8C-8A(IL125) N112A | 9.2 ± 0.9 (5) | 4.3 ± 0.3 (5) | 1.66 ± 0.10 (5) | 0.84 ± 0.06 (5) | 706 ± 243 (5) |
| | P < 0.05 | | P < 0.0001 | P < 0.05 | |
| 8C-8A(IL125) Y126F | 9.3 ± 0.3 (5) | 19.2 ± 5.9 (5) | 0.76 ± 0.07 (5) | 0.27 ± 0.05 (5) | 665 ± 120 (5) |
| | | P < 0.0001 | P < 0.0001 | P < 0.01 | |
| 8C-8A(IL125) L136E | 6.3 ± 0.5 (5) | 80.2 ± 25.4 (5) | 2.36 ± 0.08 (5) | 0.65 ± 0.09 (5) | 2,634 ± 585 (5) |
| P < 0.0005 | | P < 0.05 | | | |
| 8C-8A(IL125) L136K | 9.5 ± 0.6 (5) | 196.3 ± 54.7 (5) | 1.55 ± 0.11 (5) | 0.18 ± 0.01 (5) | 512 ± 44 (5) |
| | | | P < 0.0001 | P < 0.005 | |
| 8A + 8C | 10.8 ± 0.3 (6) | 138.3 ± 46.0 (6) | 2.41 ± 0.25 (6) | 0.53 ± 0.06 (6) | 1,698 ± 289 (6) |
| 8A E6A + 8C E6A | 11.9 ± 0.4 (5) | 96.2 ± 33.9 (5) | 3.69 ± 0.60 (5) | 0.56 ± 0.10 (5) | 1,200 ± 202 (5) |
| 8A T48D + 8C M48D | 10.1 ± 0.7 (5) | 249.7 ± 93.7 (5) | 7.28 ± 0.76 (5) | 0.65 ± 0.09 (5) | 1,661 ± 315 (5) |
| | | P < 0.0001 | | | |
| 8A + 8C L105R | 12.0 ± 0.5 (5) | 202.0 ± 82.7 (5) | 2.05 ± 0.15 (5) | 0.30 ± 0.03 (5) | 880 ± 223 (5) |
| 8A Y124F + 8C Y126F | 11.4 ± 0.1 (5) | 102.0 ± 20.9 (5) | 2.19 ± 0.08 (5) | 0.31 ± 0.04 (5) | 1,060 ± 130 (5) |
| 8A L134E + 8C L136E | 11.3 ± 0.4 (5) | 154.5 ± 55.6 (5) | 3.20 ± 0.18 (5) | 0.87 ± 0.12 (5) | 994 ± 298 (5) |
| | | | P < 0.05 | | |
| 8A L134K + 8C L136K | 11.6 ± 0.5 (5) | 119.0 ± 49.4 (5) | 2.76 ± 0.25 (5) | 0.36 ± 0.05 (5) | 1,126 ± 232 (5) |
Values are mean SEM. Rectification was calculated as the ratio of peak current measured at +120 mV and −120 mV. P values reflect comparisons of WT 8C-8A(IL125) or 8A + 8C with mutants.
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