Table 3.

qPCR primer sequences

TargetForward SequenceReverse sequence
hIFI27 5′-GCC​ACA​ACT​CCT​CCA​ATC​AC-3′ 5′-ATC​AGC​AGT​GAC​CAG​TGT​GG-3′ 
hIFI44L 5′-ACT​AAA​GTG​GAT​GAT​TGC​AG-3′ 5′-TGC​AGA​GAG​GAT​GAG​AAT​ATC-3′ 
hIFIT1 5′-ATC​CAC​AAG​ACA​GAA​TAG​CCA-3′ 5′-CCA​GAC​TAT​CCT​TGA​CCT​GAT-3′ 
hISG15 5′-ACA​GCC​ATG​GGC​TGG​GA-3′ 5′-CTT​CTG​GGT​GAT​CTG​CGC​CT-3′ 
hRSAD2 5′-CTC​TGT​GGA​GGA​GCC​TGG​TC-3′ 5′-AGT​TGA​TCT​TCT​CCA​TAC​CAG​CTT-3′ 
hSIGLEC1 5′-TGC​TCC​GTG​TGC​TCT​ACC​CT-3′ 5′-CAC​CGA​ATT​CTC​GCC​CAT​GC-3′ 
hIFNB1 5′-TGT​CGC​CTA​CTA​CCT​GTT​GTG​C-3′ 5′-AAC​TGC​AAC​CTT​TCG​AAG​CC-3′ 
hTNF 5′-TCT​CTC​AGC​TCC​ACG​CCA​TT-3′ 5′-CCC​AGG​CAG​TCA​GAT​CAT​CTT​C-3′ 
hIL6 5′-TCA​ATA​TTA​GAG​TCT​CAA​CCC​CCA-3′ 5′-GAA​GGC​GCT​TGT​GGA​GAA​GG-3′ 
hIL1B 5′-ATG​ATG​GCT​TAT​TAC​AGT​GGA​AA-3′ 5′-GTC​GGA​GAT​TCG​TAG​CTG​GA-3′ 
hACTB 5′-GCG​AGA​AGA​TGA​CCC​AGA​TC-3′ 5′-CCA​GTG​GTA​CGG​CCA​GAG​G-3′ 
hSAMHD1 exon 2–3 5′-TCA​CAG​GCG​CAT​TAC​TGC​C-3′ 5′-GGA​TTT​GAA​CCA​ATC​GCT​GGA-3′ 
hSAMHD1 exon 4–5 5′-CCT​CTC​CTC​GTC​CGA​ATC​ATT-3′ 5′-CCA​CCC​CTA​GAC​TAT​GCT​CAA-3′ 
hSAMHD1 exon 6–7 5′-GTC​ATG​GGC​CAT​TTT​CTC​AC-3′ 5′-CTG​AGC​CTT​GTT​CAT​GCG​TCC-3′ 
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