Table 1.

Reagent or resource

Reagent or resourceSourceIdentifier
Antibodies ​ ​ 
EPHA2 mouse WB 1:500 IF 1:100 SCBT sc-398832 
EPHA2 phospho Ser897 rabbit WB 1:1,000 IF 1:200 CST #6347 
EPHA2 phospho Tyr588 rabbit WB 1:1,000 CST #12677 
ASO rabbit IF 1:1,000 Ionis Pharma Ionis 13545 
MEK1/2 total rabbit WB 1:1,000 CST #9126 
MEK1/2 phospho Ser217/22 rabbit WB 1:1,000 CST #9154 
Vinculin mouse WB 1:1,000 SCBT sc-73614 
ERK1/2 total rabbit WB 1:1,000 Sigma-Aldrich M5670 
ERK1/2 phospho mouse WB 1:1,000 Sigma-Aldrich M8159 
KRAS mouse WB 1:1,000 LSBio LS-C175665 
p90RSK phospho S380 rabbit WB 1:1,000 CST #9341 
GAPDH mouse WB 1:1,000 SCBT sc-365062 
RPS6 phospho Ser235/236 rabbit WB 1:1,000 CST #4858 
CD44 rabbit WB 1:1,000 IF 1:100 CST #37259 
CD44-AF647 IF 1:500 BioLegend 103017 
CD44 (for Duolink PLA) BioLegend 103001 
streptavidin 488 IF 1:400 Thermo Fisher Scientific S11223 
eIF2a phospho Ser51 rabbit WB 1:500 Abcam ab32157 
eIF2a mouse WB 1:1,000 CST #2103 
beta-Actin mouse WB 1:5,000 Sigma-Aldrich AC-74 
Histone H3 rabbit WB 1:1,000 CST #4499 
AKT phospho Ser473 rabbit WB 1:1,000 CST #4060 
JNK phospho Y16/T221 rabbit WB 1:1,000 CST #9251 
p38MAPK phospho T180/Y182 rabbit WB 1:1,000 CST #4511 
SCARB1 rabbit WB 1:1,000 IF 1:100 Proteintech 212277-1-AP 
beta-Tubulin mouse WB 1:5,000 Sigma-Aldrich T8328 
G3BP1 rabbit WB 1:1,000 Proteintech 13057-2-AP 
G3BP2 rabbit WB 1:1,000 Proteintech 16276-1-AP 
eIF3b mouse IF 1:100 SCBT sc-137214 
IRDye 680RD Donkey anti-Mouse IgG Secondary Antibody donkey WB 1:5,000 Licor Bio 926-68072 
IRDye 800CW Donkey anti-Rabbit IgG Secondary Antibody donkey WB 1:5,000 Licor Bio 926-32213 
Anti-mouse IgG, HRP-linked Antibody horse WB 1:5,000 CST #7076 
Anti-rabbit IgG, HRP-linked Antibody goat WB 1:5,000 CST #7074 
Donkey anti-Mouse IgG (H+L) highly cross-Adsorbed Secondary Antibody, Alexa Fluor 488 donkey IF 1:400 Thermo Fisher Scientific A21202 
Donkey anti-Mouse IgG (H+L) highly cross-Adsorbed Secondary Antibody, Alexa Fluor 568 donkey IF 1:400 Thermo Fisher Scientific A10037 
Donkey anti-Mouse IgG (H+L) highly cross-Adsorbed Secondary Antibody, Alexa Fluor 647 donkey IF 1:400 Thermo Fisher Scientific A31571 
Donkey anti-Rabbit IgG (H+L) highly cross-Adsorbed Secondary Antibody, Alexa Fluor 488 donkey IF 1:400 Thermo Fisher Scientific A21206 
Donkey anti-Rabbit IgG (H+L) highly cross-Adsorbed Secondary Antibody, Alexa Fluor 568 donkey IF 1:400 Thermo Fisher Scientific A10042 
Donkey anti-Rabbit IgG (H+L) highly cross-Adsorbed Secondary Antibody, Alexa Fluor 647 donkey IF 1:400 Thermo Fisher Scientific A31573 
Bacterial and virus strains ​ ​ 
One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific C737303 
Chemicals, peptides, and recombinant proteins ​ ​ 
Lipofectamine 2000 Thermo Fisher Scientific 11668027 
Nucleofection Kit V Lonza VCA-1003 
Quantinova SYBR Green RT-PCR Kit Qiagen 208352 
cET-ASOKRASHomo sapiens Ionis Pharma ​ 
cET-ASONT Ionis Pharma ​ 
RSKi – LJH685 selleckchem S7870 
Biotin Sigma-Aldrich B4501 
Streptavidin-agarose beads Millipore 16-126 
Poly(dT) probe Biosearch Technologies Stellaris positive Control T30-CalFluor 590 
ISRIB MedchemExpress HY-12495 
BafilomycinA1 Cell Signaling #54645 
Chloroquine diphosphate salt Merck Sigma-Aldrich C6628 
Recombinant human CD44 protein (ECD) Sino Biologicals 12211-HNAH 
Hyaluronate CyDye, MW 10 kDa HAWORKS USA HA-CyDye-10k 
HA, MW 10 kDa HAWORKS USA HA-10k 
Hoechst 33342 Thermo Fisher Scientific H3570 
CellROX green Thermo Fisher Scientific C10444 
C11-bodipy 581/591 Thermo Fisher Scientific D3861 
Phalloidin-Alexa488 Thermo Fisher Scientific A12379 
Commercial kits ​ ​ 
Duolink In Situ Detection Reagents Red Merck Sigma-Aldrich DUO92008 
Duolink In Situ PLA probe Anti-Rabbit PLUS Merck Sigma-Aldrich DUO92002 
Duolink In Situ Probemaker MINUS Merck Sigma-Aldrich DUO92010 
Duolink In Situ Wash Buffers, Fluorescence Merck Sigma-Aldrich DUO82049 
Deposited data ​ ​ 
H1299 expression of SRrs - GSE179901 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE179901 Data deposited in Marco et al. (2021)  
Experimental models: Cell lines ​ ​ 
NCI-H1299 cell line ATCC CRL-5803 
HEK293FT Thermo Fisher Scientific R70007 
KPC wild-type mouse tumor-derived cell line Beatson Institute As described in Gundry et al. (2017)  
KPC Epha2−/− mouse tumor-derived cell line Beatson Institute ​ 
Oligonucleotides (cET-ASOs) ​ ​ 
3-10-3 cEt for MALAT1 (mouse or human) Ionis Pharmaceuticals 556089 
3-10-3 cEt nontargeting ASO (mouse or human) Ionis Pharmaceuticals 549148 
3-10-3 cEt for human KRAS Ionis Pharmaceuticals 651987 (a.k.a. AZD4785) 
Oligonucleotides (other) ​ ​ 
Rab17 sgRNA #1 (5′-3′ sense/antisense) 5′-TCC​GAG​TGA​TGT​CAT​AAA​CC/GGT​TTA​TGA​CAT​CAC​TCG​GA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Rab17 sgRNA #2 (5′-3′ sense/antisense) 5′-AGA​ACC​AGC​TTG​CTC​ACG​TA/TAC​GTG​AGC​AAG​CTG​GTT​CT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Rab17 sgRNA #3 (5′-3′ sense/antisense) 5′-CCT​CAC​TTA​CAC​CCC​ACA​GT/ACT​GTG​GGG​TGT​AAG​TGA​GG-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Rab17 sgRNA #4 (5′-3′ sense/antisense) 5′-CAT​TGG​CAC​CCC​TGA​AGT​AG/CTA​CTT​CAG​GGG​TGC​CAA​TG-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cd44 sgRNA #1 (5′-3′ sense/antisense) 5′-AAT​GTA​ACC​TGC​CGC​TAC​GC/GCG​TAG​CGG​CAG​GTT​ACA​TT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cd44 sgRNA #2 (5′-3′ sense/antisense) 5′-TGG​GTT​CAT​AGA​AGG​AAA​TG/CAT​TTC​CTT​CTA​TGA​ACC​CA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cd44 sgRNA #3 (5′-3′ sense/antisense) 5′-AGG​AGA​TCG​AGA​CTC​ATC​CA/TGG​ATG​AGT​CTC​GAT​CTC​CT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cd44 sgRNA #4 (5′-3′ sense/antisense) 5′-TAT​GGT​AAC​CGG​TCC​ATC​GA/TCG​ATG​GAC​CGG​TTA​CCA​TA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Scarb1 sgRNA #1 (5′-3′ sense/antisense) 5′-GGG​GCC​GTG​AAG​CGA​TAC​GT/CGT​ATC​GCT​TCA​CGG​CCC​C-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Scarb1 sgRNA #2 (5′-3′ sense/antisense) 5′-GAG​GAT​TCG​GGT​GTC​ATG​AA/TTC​ATG​ACA​CCC​GAA​TCC​TC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Scarb1 sgRNA #3 (5′-3′ sense/antisense) 5′-TCT​GAG​CCA​TGC​GAC​TTG​TC/GAC​AAG​TCG​CAT​GGC​TCA​GA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Scarb1 sgRNA #4 (5′-3′ sense/antisense) 5′-GCT​GAT​GAT​GAC​CTT​GGC​GC/GCG​CCA​AGG​TCA​TCA​TCA​GC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Epha2 sgRNA #1 (5′-3′ sense/antisense) 5′-GCT​GAC​CGT​GAT​CTC​GTC​AG/CTG​ACG​AGA​TCA​CGG​TCA​GC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Epha2 sgRNA #2 (5′-3′ sense/antisense) 5′-TCG​GTG​TGC​AAC​GTG​GTA​TC/GAT​ACC​ACG​TTG​CAC​ACC​GA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Epha2 sgRNA #3 (5′-3′ sense/antisense) 5′-GGT​CTA​TAA​AGG​GAC​GCT​GA/TCA​GCG​TCC​CTT​TAT​AGA​CC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Epha2 sgRNA #4 (5′-3′ sense/antisense) 5′-TGT​CTC​CGA​TAC​ACA​ACC​CC/GGG​GTT​GTG​TAT​CGG​AGA​CA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
G3bp1 sgRNA #1 (5′-3′ sense/antisense) 5′-GCA​GTC​TAC​GGG​CAG​AAG​GT/ACC​TTC​TGC​CCG​TAG​ACT​GC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
G3bp1 sgRNA #2 (5′-3′ sense/antisense) 5′-AGG​CCC​CGG​ACA​TGT​TGC​AC/GTG​CAA​CAT​GTC​CGG​GGC​CT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
G3bp1 sgRNA #3 (5′-3′ sense/antisense) 5′-ACT​ACA​GAG​CAA​CAT​ACC​TC/GAG​GTA​TGT​TGC​TCT​GTA​GT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
G3bp1 sgRNA #4 (5′-3′ sense/antisense) 5′-TGT​GCA​GAA​GAG​CAC​TTC​CC/GGG​AAG​TGC​TCT​TCT​GCA​CA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
G3bp2 sgRNA #1 (5′-3′ sense/antisense) 5′-ATC​CAC​TCC​ACC​ATG​AAC​AT/ATG​TTC​ATG​GTG​GAG​TGG​AT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
G3bp2 sgRNA #2 (5′-3′ sense/antisense) 5′-GCT​AGC​TAG​CCT​TAC​GTC​AC/GTG​ACG​TAA​GGC​TAG​CTA​GC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
G3bp2 sgRNA #3 (5′-3′ sense/antisense) 5′-CCG​GAT​AGC​GAA​TTA​TTC​TC/GAG​AAT​AAT​TCG​CTA​TCC​GG-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
G3bp2 sgRNA #4 (5′-3′ sense/antisense) 5′-TCT​GGC​TTA​GCA​TCA​ACC​CT/AGG​GTT​GAT​GCT​AAG​CCA​GA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
EPHA2 sgRNA #1 (5′-3′ sense/antisense) 5′-TCC​GTG​TGC​AAC​GTG​ATG​TC/GAC​ATC​ACG​TTG​CAC​ACG​GA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
EPHA2 sgRNA #2 (5′-3′ sense/antisense) 5′-GTG​AAG​GTG​TAG​TTC​ATG​TG/CAC​ATG​AAC​TAC​ACC​TTC​AC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
EPHA2 sgRNA #3 (5′-3′ sense/antisense) 5′-CAA​GTT​GCC​AGA​TCC​CTC​CG/CGG​AGG​GAT​CTG​GCA​ACT​TG-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
EPHA2 sgRNA #4 (5′-3′ sense/antisense) 5′-CAC​ATA​TGA​GGA​CCC​CAA​CC/GGT​TGG​GGT​CCT​CAT​ATG​TG-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
KRAS real-time PCR primers (5′-3′ forward/reverse) 5′-ACA​GAG​AGT​GGA​GGA​TGC​TTT/TTT​CAC​ACA​GCC​AGG​AGT​CTT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Kras real-time PCR primers (5′-3′ forward/reverse) 5′-CAA​GAG​CGC​CTT​GAC​GAT​ACA​CCA​AGA​GAC​AGG​TTT​CTC​CAT​C-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
18S real-time PCR primers (5′-3′ forward/reverse) 5′-GCT​TAA​TTT​GAC​TCA​ACA​CGG​GAA​GCT​ATC​AAT​CTG​TCA​ATC​CTG​TC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Msr1 real-time PCR primers (5′-3′ forward/reverse) 5′-GCA​CAA​TCT​GTG​ATG​ATC​GCT/CCC​AGC​ATC​TTC​TGA​ATG​TGA​A-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Colec12 real-time PCR primers (5′-3′ forward/reverse) 5′-GGT​GCA​GTC​CTT​CGG​TTA​CAA/GAT​GGT​CAG​TAA​GGC​ACA​CAG-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Scara5 real-time PCR primers (5′-3′ forward/reverse) 5′-CAT​GGA​TTT​CAC​AAT​GAT​TCG​CC/TCC​CCG​TCC​TTC​TTG​TCC​C-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Marco real-time PCR primers (5′-3′ forward/reverse) 5′-ACA​GAG​CCG​ATT​TTG​ACC​AAG/CAG​CAG​TGC​AGT​ACC​TGC​C-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Scarb1 real-time PCR primers (5′-3′ forward/reverse) 5′-TTT​GGA​GTG​GTA​GTA​AAA​AGG​GC/TGA​CAT​CAG​GGA​CTC​AGA​GTA​G-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cd36 real-time PCR primers (5′-3′ forward/reverse) 5′-AGA​TGA​CGT​GGC​AAA​GAA​CAG/CCT​TGG​CTA​GAT​AAC​GAA​CTC​TG-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cd68 real-time PCR primers (5′-3′ forward/reverse) 5′-TGT​CTG​ATC​TTG​CTA​GGA​CCG/GAG​AGT​AAC​GGC​CTT​TTT​GTG​A-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Olr1 real-time PCR primers (5′-3′ forward/reverse) 5′-CAA​GAT​GAA​GCC​TGC​GAA​TGA/ACC​TGG​CGT​AAT​TGT​GTC​CAC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Clec7a real-time PCR primers (5′-3′ forward/reverse) 5′-GAC​TTC​AGC​ACT​CAA​GAC​ATC​C/TTG​TGT​CGC​CAA​AAT​GCT​AGG-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Mrc1 real-time PCR primers (5′-3′ forward/reverse) 5′-CTC​TGT​TCA​GCT​ATT​GGA​CGC/CGG​AAT​TTC​TGG​GAT​TCA​GCT​TC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Asgr1 real-time PCR primers (5′-3′ forward/reverse) 5′-TGA​GCA​CCC​AGG​GAA​GTA​GT/CCA​TTG​CCC​CGA​AAT​GCA​G-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Scarf1 real-time PCR primers (5′-3′ forward/reverse) 5′-TGG​GAC​TAG​AGC​TGG​TGT​TCT/CAG​ATG​GGG​ATG​GTG​CAT​TCT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Megf10 real-time PCR primers (5′-3′ forward/reverse) 5′-CCC​TCA​CTG​TGC​TGA​TAA​ATG​T/TGA​TGG​GGT​TAC​ACA​AAG​CTC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cxcl16 real-time PCR primers (5′-3′ forward/reverse) 5′-CCT​TGT​CTC​TTG​CGT​TCT​TCC/TCC​AAA​GTA​CCC​TGC​GGT​ATC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Stab1 real-time PCR primers (5′-3′ forward/reverse) 5′-GGC​AGA​CGG​TAC​GGT​CTA​AAC/AGC​GGC​AGT​CCA​GAA​GTA​TCT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Stab2 real-time PCR primers (5′-3′ forward/reverse) 5′-AGC​TGC​TGC​CTT​TAA​TCC​TCA/ACT​CCG​TCT​TGA​TGG​TTA​GAG​TA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cd163 real-time PCR primers (5′-3′ forward/reverse) 5′-ATG​GGT​GGA​CAC​AGA​ATG​GTT/CAG​GAG​CGT​TAG​TGA​CAG​CAG-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cd163l1 real-time PCR primers (5′-3′ forward/reverse) 5′-CTG​GCC​TCT​GAG​TTT​AGG​GTC/CCC​TTG​GTG​TCG​AAC​CAG​C-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Mscart1 real-time PCR primers (5′-3′ forward/reverse) 5′-CCC​TTG​GTG​TCG​AAC​CAG​C/GTG​GCT​GTG​ATT​ACC​TGC​TCT-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Scart2 real-time PCR primers (5′-3′ forward/reverse) 5′-TTC​CCG​TTA​GTT​CCA​TCG​TGG/GGA​GAA​ATG​GCT​TTC​AAC​CTG​A-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Ager real-time PCR primers (5′-3′ forward/reverse) 5′-CTT​GCT​CTA​TGG​GGA​GCT​GTA/GGA​GGA​TTT​GAG​CCA​CGC​T-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Cd44 real-time PCR primers (5′-3′ forward/reverse) 5′-TCG​ATT​TGA​ATG​TAA​CCT​GCC​G/CAG​TCC​GGG​AGA​TAC​TGT​AGC-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Lrp1 real-time PCR primers (5′-3′ forward/reverse) 5′-ACT​ATG​GAT​GCC​CCT​AAA​ACT​TG/GCA​ATC​TCT​TTC​ACC​GTC​ACA-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
Lrp2 real-time PCR primers (5′-3′ forward/reverse) 5′-AAA​ATG​GAA​ACG​GGG​TGA​CTT/GGC​TGC​ATA​CAT​TGG​GTT​TTC​A-3′ Thermo Fisher Scientific Custom 25 nM desalted primer 
siRNA ​ ​ 
CD44 Horizon Discovery L-009999-00-0005 
SCARB1 Horizon Discovery L-010592-00-0005 
LRP1 Horizon Discovery L-004721-00-0005 
Recombinant DNA​
RAB17 - RAB17-mCherry As described by Diaz-Vera et al. (2017)  
EPHA2WT - EPHA2 WT-GFP As described by Gundry et al. (2017)  
EPHA2S897A - EPHA2 phospho-defective S897-GFP ​ 
EPHA2NLS - EPHA2 K583A K586A-GFP As described by Marco et al. (2021)  
EPHA2-turboID - EPHA2 WT-TurboID-T2A-BFP H. sapiens ​ 
Galectin-9 - Galectin-9-mCherry Addgene #166689 
G3BP1 - G3BP1-GFP From Gerald McInerney, Karolinska Institute, as described by Panas et al. (2015)  
RAB11 – Rab11-mCherry As described by Gundry et al. (2017)  
RAB14 – Rab14-mCherry ​ 
RCP – RCP-mCherry ​ 
Software and algorithms ​ 
Zeiss Zen (black edition) system 2.3 Carl Zeiss Microscopy 
Zeiss Zen (blue edition) 2.3 Carl Zeiss Microscopy 
Imaris 10.1.1 Oxford Instruments 
ImageJ – Fiji 2.6.0 As described by Schindelin et al. (2012)  
GraphPad Prism 10.3.1 GraphPad Software LLC 
CFX Maestro Software for CFX Real-Time PCR 2.3 Bio-Rad 
Image Studio Software 6.0 Li-Cor 
Harmony High-Content Imaging and Analysis Software 4.9 Revvity 
Columbus Image Data Storage and Analysis System 2.9.1 Revvity 
R version 4.5.0 (2025-04-11 ucrt) Posit Software, PBC 
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