Primer sequences and efficiencies for qPCR
| Gene | Primer | Sequence | Average efficiency | Source |
|---|---|---|---|---|
| αTub84B | Forward | 5′-TGTCGCGTGTGAAACACTTC-3′ | 94.4% | Ponton et al. (2011) |
| Reverse | 5′-AGCAGGCGTTTCCAATCTG-3′ | |||
| eEF1α2 | Forward | 5′-GCGTGGGTTTGTGATCAGTT-3′ | 99.3% | |
| Reverse | 5′-GATCTTCTCCTTGCCCATCC-3′ | |||
| cid | Forward | 5′-GAAGACGGCACCGACTACGG-3′ | 95.5% | Collins et al. (2018) |
| Reverse | 5′-CGTCGAGGAACGCCGATTGT-3′ | |||
| Cenp-C | Forward | 5′-AGGAGACCGTAAACTTGACCCG-3′ | 97.7% | |
| Reverse | 5′-TTCTCTGTGCAAGGTGTGCTG-3′ | |||
| cal1 | Forward | 5′-AATGAGGATAACGAGGCAAAG-3′ | 97.8% | |
| Reverse | 5′-TGTCCAGAATGCGATCCAG-3′ |
| Gene | Primer | Sequence | Average efficiency | Source |
|---|---|---|---|---|
| Forward | 5′-TGTCGCGTGTGAAACACTTC-3′ | 94.4% | ||
| Reverse | 5′-AGCAGGCGTTTCCAATCTG-3′ | |||
| Forward | 5′-GCGTGGGTTTGTGATCAGTT-3′ | 99.3% | ||
| Reverse | 5′-GATCTTCTCCTTGCCCATCC-3′ | |||
| Forward | 5′-GAAGACGGCACCGACTACGG-3′ | 95.5% | ||
| Reverse | 5′-CGTCGAGGAACGCCGATTGT-3′ | |||
| Forward | 5′-AGGAGACCGTAAACTTGACCCG-3′ | 97.7% | | |
| Reverse | 5′-TTCTCTGTGCAAGGTGTGCTG-3′ | |||
| Forward | 5′-AATGAGGATAACGAGGCAAAG-3′ | 97.8% | | |
| Reverse | 5′-TGTCCAGAATGCGATCCAG-3′ |
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