Table 1.

List of primers used for PCR1 to amplify guide RNA sequences from genomic DNA

PrimerSequence
CRISPR NGS F1 5′-TCG​TCG​GCA​GCG​TCA​GAT​GTG​TAT​AAG​AGA​CAG​TGT​GGA​AAG​GAC​GAA​ACA​CC-3′ 
CRISPR NGS F1.1 5′-TCG​TCG​GCA​GCG​TCA​GAT​GTG​TAT​AAG​AGA​CAG​NTG​TGG​AAA​GGA​CGA​AAC​ACC-3′ 
CRISPR NGS F1.2 5′-TCG​TCG​GCA​GCG​TCA​GAT​GTG​TAT​AAG​AGA​CAG​NNT​GTG​GAA​AGG​ACG​AAA​CAC​C-3′ 
CRISPR NGS F1.3 5′-TCG​TCG​GCA​GCG​TCA​GAT​GTG​TAT​AAG​AGA​CAG​NNN​TGT​GGA​AAG​GAC​GAA​ACA​CC-3′ 
CRISPR NGS F1.4 5′-TCG​TCG​GCA​GCG​TCA​GAT​GTG​TAT​AAG​AGA​CAG​NNN​NTG​TGG​AAA​GGA​CGA​AAC​ACC-3′ 
CRISPR NGS F1.5 5′-TCG​TCG​GCA​GCG​TCA​GAT​GTG​TAT​AAG​AGA​CAG​NNN​NNT​GTG​GAA​AGG​ACG​AAA​CAC​C-3′ 
CRISPR NGS R4a 5′-GTC​TCG​TGG​GCT​CGG​AGA​TGT​GTA​TAA​GAG​ACA​GCT​AGA​GCC​ATT​TGT​CTG​CAG​A-3′ 

or Create an Account

Close Modal
Close Modal