Table 5. Parameters from activation GVs,... | Rockefeller University Press

Table 5.

Parameters from activation GVs, inactivation time constants, and SSI curves for FGF14A mutant constructs and FGF14B

Constructs	GV curve				Inactivation τ (0 mV)
Constructs	V_h (mV)	P value	z (e)	P value	τ_i (ms)	P value	N
Na_V1.2	−17.1 ± 6.5		4.35 ± 1.49		0.36 ± 0.09		14 (8)
+FGF14A	−20.3 ± 4.2	0.69 ^WT	4.47 ± 0.88	>0.99 ^WT	0.58 ± 0.12	<0.0001 ^WT	15 (10)
+FGF14A_LI/AA	−25.3 ± 2.2	0.01 ^WT 0.07 ^14A	4.81 ± 0.81	0.98 ^WT 0.99 ^14A	0.57 ± 0.03	<0.0001 ^WT >0.99 ^14A	4 (3)
+FGF14A_2Q	−22.0 ± 1.6	0.15 ^WT 0.84 ^14A	5.20 ± 0.68	0.64 ^WT 0.57 ^14A	0.47 ± 0.03	0.009 ^WT 0.03 ^14A	4 (2)
+FGF14A_5Q	−22.6		5.65		0.78		1 (1)
+FGF14A_ΔNT	−19.5 ± 4.6	0.97 ^WT >0.99 ^14A	4.72 ± 1.30	0.99 ^WT 0.99 ^14A	0.30 ± 0.05	0.61 ^WT <0.0001 ^14A	5 (3)
+FGF14B	−22.6 ± 3.2	0.09 ^WT 0.64 ^14A	4.94 ± 0.55	0.79 ^WT 0.60 ^14A	0.64 ± 0.09	<0.0001 ^WT 0.72 ^14A	12 (4)
	SSI curve
	V_h (mV)		P value	z (e)		P value	N
Na_V1.2	−62.4 ± 3.5			4.51 ± 0.50			22 (16)
+FGF14A	−48.2 ± 2.6		<0.0001 ^WT	5.85 ± 0.64		<0.0001 ^WT	31 (18)
+FGF14A_LI/AA	−47.4 ± 3.0		<0.0001 ^WT >0.99 ^14A	5.54 ± 0.37		0.007 ^WT >0.99 ^14A	6 (2)
+FGF14A_2Q	−47.6 ± 1.2		<0.0001 ^WT >0.99 ^14A	6.36 ± 0.78		<0.0001 ^WT >0.99 ^14A	5 (2)
+FGF14A_5Q	−46.2 ± 2.2		<0.0001 ^WT >0.99 ^14A	5.62 ± 1.07		0.06 ^WT >0.99 ^14A	3 (2)
+FGF14A_ΔNT	−57.5 ± 1.2		0.004 ^WT <0.0001 ^14A	5.19 ± 0.40		0.23 ^WT 0.21 ^14A	6 (3)
+FGF14B	−52.9 ± 3.2		<0.0001 ^WT <0.0001 ^14A	5.23 ± 0.74		0.02 ^WT 0.04 ^14A	13 (5)

Constructs	GV curve				Inactivation τ (0 mV)
Constructs	V_h (mV)	P value	z (e)	P value	τ_i (ms)	P value	N
Na_V1.2	−17.1 ± 6.5		4.35 ± 1.49		0.36 ± 0.09		14 (8)
+FGF14A	−20.3 ± 4.2	0.69 ^WT	4.47 ± 0.88	>0.99 ^WT	0.58 ± 0.12	<0.0001 ^WT	15 (10)
+FGF14A_LI/AA	−25.3 ± 2.2	0.01 ^WT0.07 ^14A	4.81 ± 0.81	0.98 ^WT0.99 ^14A	0.57 ± 0.03	<0.0001 ^WT>0.99 ^14A	4 (3)
+FGF14A_2Q	−22.0 ± 1.6	0.15 ^WT0.84 ^14A	5.20 ± 0.68	0.64 ^WT0.57 ^14A	0.47 ± 0.03	0.009 ^WT0.03 ^14A	4 (2)
+FGF14A_5Q	−22.6		5.65		0.78		1 (1)
+FGF14A_ΔNT	−19.5 ± 4.6	0.97 ^WT>0.99 ^14A	4.72 ± 1.30	0.99 ^WT0.99 ^14A	0.30 ± 0.05	0.61 ^WT<0.0001 ^14A	5 (3)
+FGF14B	−22.6 ± 3.2	0.09 ^WT0.64 ^14A	4.94 ± 0.55	0.79 ^WT0.60 ^14A	0.64 ± 0.09	<0.0001 ^WT0.72 ^14A	12 (4)
	SSI curve
	V_h (mV)		P value	z (e)		P value	N
Na_V1.2	−62.4 ± 3.5			4.51 ± 0.50			22 (16)
+FGF14A	−48.2 ± 2.6		<0.0001 ^WT	5.85 ± 0.64		<0.0001 ^WT	31 (18)
+FGF14A_LI/AA	−47.4 ± 3.0		<0.0001 ^WT>0.99 ^14A	5.54 ± 0.37		0.007 ^WT>0.99 ^14A	6 (2)
+FGF14A_2Q	−47.6 ± 1.2		<0.0001 ^WT>0.99 ^14A	6.36 ± 0.78		<0.0001 ^WT>0.99 ^14A	5 (2)
+FGF14A_5Q	−46.2 ± 2.2		<0.0001 ^WT>0.99 ^14A	5.62 ± 1.07		0.06 ^WT>0.99 ^14A	3 (2)
+FGF14A_ΔNT	−57.5 ± 1.2		0.004 ^WT<0.0001 ^14A	5.19 ± 0.40		0.23 ^WT0.21 ^14A	6 (3)
+FGF14B	−52.9 ± 3.2		<0.0001 ^WT<0.0001 ^14A	5.23 ± 0.74		0.02 ^WT0.04 ^14A	13 (5)

Statistical analyses were performed using Welch’s ANOVA test, followed by Dunnett’s T3 multiple comparisons test (for GV data) or one-way ANOVA, followed by Bonferroni’s multiple comparisons test (for SSI data).

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