Time constants of inactivation onset and recovery for NaV1.2_IQM expressed with FGF A-isoforms
| Constructs | Inactivation τ (0 mV) | Slow recovery from inactivation (−80 mV) | |||||
|---|---|---|---|---|---|---|---|
| τi (ms) | P value | N | τs (ms) | P value | As | N | |
| NaV1.2 | 0.36 ± 0.09 (Table S1) | | 14 | | | | |
| NaV1.2_IQM | | | | | | | |
| +FGF14A | 0.92 ± 0.24 | 0.005 13A 0.02 12A 0.99 11A | 5 (5) | 565.5 ± 88.3 | <0.0001 13A >0.99 12A <0.0001 11A | 0.98 ± 0.03 | 4 (4) |
| +FGF13A | 1.50 ± 0.30 | 0.64 12A 0.002 11A | 4 (3) | 1831.5 ± 383.5 | <0.0001 12A <0.0001 11A | 0.95 ± 0.03 | 6 (4) |
| +FGF12A | 1.33 ± 0.20 | 0.008 11A | 7 (5) | 565.4 ± 112.5 | <0.0001 11A | 1.00 ± 0.03 | 10 (6) |
| +FGF11A | 0.88 ± 0.16 | | 6 (4) | 155.8 ± 34.5 | | 0.97 ± 0.02 | 10 (4) |
| Constructs | Inactivation τ (0 mV) | Slow recovery from inactivation (−80 mV) | |||||
|---|---|---|---|---|---|---|---|
| τi (ms) | P value | τs (ms) | P value | As | |||
| NaV1.2 | 0.36 ± 0.09 ( | | 14 | | | | |
| NaV1.2_IQM | | | | | | | |
| +FGF14A | 0.92 ± 0.24 | 0.005 13A | 5 (5) | 565.5 ± 88.3 | <0.0001 13A | 0.98 ± 0.03 | 4 (4) |
| +FGF13A | 1.50 ± 0.30 | 0.64 12A | 4 (3) | 1831.5 ± 383.5 | <0.0001 12A | 0.95 ± 0.03 | 6 (4) |
| +FGF12A | 1.33 ± 0.20 | 0.008 11A | 7 (5) | 565.4 ± 112.5 | <0.0001 11A | 1.00 ± 0.03 | 10 (6) |
| +FGF11A | 0.88 ± 0.16 | | 6 (4) | 155.8 ± 34.5 | | 0.97 ± 0.02 | 10 (4) |
Statistical analyses were performed using one-way ANOVA, followed by Tukey’s multiple comparisons test (for τslow parameter, a lognormal distribution was used to satisfy ANOVA assumptions of normality and homogeneity of variance). Rank order of derived rate constants.
klti: FGF11A ∼ FGF14A > FGF12A > FGF13A.
k-lti: FGF11A > FGF12A ∼ FGF14A > FGF13A.