Immune function testing results
| Test . | Reference range . | Age 8 wk . | Age 17 wk . | Age 20 wk . |
|---|---|---|---|---|
| Complete blood count | ||||
| White blood cell count (K/mcL) | 6.5–13.3 | 14.2 | 17.5 | 30.2 |
| Hemoglobin (g/dl) | 9.6–12.4 | 7.3 | 9.3 | 9.9 |
| Platelets (K/mcL) | 244–529 | 206 | 499 | 342 |
| Absolute neutrophil count (K/mcL) | 0.97-5–45 | 4.5 | 9.5 | 28.1 |
| Absolute lymphocyte count (K/mcL) | 2.45–8.89 | 8.4 | 6.8 | 1.2 |
| Absolute monocyte count (K/mcL) | 0.28-1.07 | 0.3 | 1.2 | 0.9 |
| Absolute eosinophil count (K/mcL) | 0.03–0.61 | 0.7 | 0 | 0 |
| Lymphocyte immunophenotyping by flow cytometry | ||||
| Absolute CD3+ T cell count (cells/mcL) | 2,200–9,200 | 5,869 | 5,674 | |
| Absolute CD4+ count (cells/mcL) | 1,600–6,500 | 3,422 | 4,260 | |
| Absolute CD4+/CD45RA+ count (cells/mcL) | 1,200–5,300 | 2,232 | 2,371 | |
| Absolute CD4+/CD45RO+ count (cells/mcL) | 90–1,400 | 1,303 | 1,830 | |
| Absolute CD8+ count (cells/mcL) | 300–3,400 | 2,186 | 1,354 | |
| Absolute CD19+ B cell count (cells/mcL) | 520–2,300 | 401 | 423 | |
| Absolute NK cell count (cells/mcL) | 97–2,000 | 555 | 469 | |
| FOXP3 protein expression | ||||
| Treg CD4+CD25+CD127− (% of CD4) | 4.2–9.9 | 5.5 | | |
| Absolute Treg CD4+CD25+CD127− (cells/mcL) | 43–782 | 143 | | |
| Foxp3 (% of Tregs) | 55–81 | 32 | | |
| Absolute FOXP3 (cells/mcL) | 43–782 | 46 | | |
| Immunoglobulins | ||||
| IgG (mg/dl) | 100–334 | 205 | 501 | 906 |
| IgA (mg/dl) | 7–37 | 18 | | |
| IgM(mg/dl) | 26–122 | 19 | | |
| IgE (IU/ml) | <=13 | 9,309 | 4,997 | 31,478 |
| Serum cytokines | ||||
| IL-1 β (pg/ml) | <=6.7 | <6.5 | <6.5 | <6.5 |
| IL-2 (pg/ml) | <=2.1 | <2.1 | <2.1 | <2.1 |
| IL-2 receptor, soluble (pg/ml) | 175.3–858.2 | 6,111.5 | 3,787 | 2,322 |
| IL-4 (pg/ml) | <=2.2 | <2.2 | <2.2 | <2.2 |
| IL-5 (pg/ml) | <=2.1 | 6.1 | 2.2 | <2.1 |
| IL-6 (pg/ml) | <=2.0 | 49.6 | 2.8 | 8.1 |
| IL-8 (pg/ml) | <=3.0 | 278.1 | <3.0 | 8.4 |
| IL-10 (pg/ml) | <=2.8 | 48.7 | 10.6 | 10.6 |
| IL-12 (pg/ml) | <=1.9 | <1.9 | <1.9 | <1.9 |
| IL-13 (pg/ml) | <=2.3 | 2.9 | <1.7 | 2.3 |
| IL-17 (pg/ml) | <=1.4 | 1.5 | 44.7 | 12.8 |
| IL-18 (pg/ml) | <=477 | 1,685 | | |
| Interferon-γ (pg/ml) | <=4.2 | 5.6 | <4.2 | <4.2 |
| TNF-α (pg/ml) | <=7.2 | 5.4 | 2.2 | 3.8 |
| CXCL9 (pg/ml) | <=647 | 9,535 | | |
| C-reactive protein (CRP) (mg/dl) | <0.3 | 19.2 | 5.5 | 10.9 |
| Erythrocyte Sedimentation rate (ESR) (mm/h) | 0–9 | 14 | 20 | 4 |
| Procalcitonin (ng/ml) | 0–0.09 | 0.31 | | 14.6 |
| Fecal calprotectin (mcg/g) | | 457 | 1,200 | 1,380 |
| Fecal pancreatic elastase (ug/g) | > 200 | 10 | 36 | |
| Autoantibodies | ||||
| Glutamate decarboxylase antibody (IU/ml) | 0–5.0 | <5.0 (negative) | | |
| Islet antigen-2 serum autoantibody (unit/ml) | 0–7.4 | <5.4 (negative) | | |
| Insulin antibody (unit/ml) | 0–0.4 | <0.4 (negative) | | |
| Islet cell cytoplasmic antibody, IgG | <1:4 | <1:4 (negative) | | |
| Zinc transporter 8 antibody (unit/ml) | 0–15.0 | <10.0 (negative) | | |
| Test . | Reference range . | Age 8 wk . | Age 17 wk . | Age 20 wk . |
|---|---|---|---|---|
| Complete blood count | ||||
| White blood cell count (K/mcL) | 6.5–13.3 | 14.2 | 17.5 | 30.2 |
| Hemoglobin (g/dl) | 9.6–12.4 | 7.3 | 9.3 | 9.9 |
| Platelets (K/mcL) | 244–529 | 206 | 499 | 342 |
| Absolute neutrophil count (K/mcL) | 0.97-5–45 | 4.5 | 9.5 | 28.1 |
| Absolute lymphocyte count (K/mcL) | 2.45–8.89 | 8.4 | 6.8 | 1.2 |
| Absolute monocyte count (K/mcL) | 0.28-1.07 | 0.3 | 1.2 | 0.9 |
| Absolute eosinophil count (K/mcL) | 0.03–0.61 | 0.7 | 0 | 0 |
| Lymphocyte immunophenotyping by flow cytometry | ||||
| Absolute CD3+ T cell count (cells/mcL) | 2,200–9,200 | 5,869 | 5,674 | |
| Absolute CD4+ count (cells/mcL) | 1,600–6,500 | 3,422 | 4,260 | |
| Absolute CD4+/CD45RA+ count (cells/mcL) | 1,200–5,300 | 2,232 | 2,371 | |
| Absolute CD4+/CD45RO+ count (cells/mcL) | 90–1,400 | 1,303 | 1,830 | |
| Absolute CD8+ count (cells/mcL) | 300–3,400 | 2,186 | 1,354 | |
| Absolute CD19+ B cell count (cells/mcL) | 520–2,300 | 401 | 423 | |
| Absolute NK cell count (cells/mcL) | 97–2,000 | 555 | 469 | |
| FOXP3 protein expression | ||||
| Treg CD4+CD25+CD127− (% of CD4) | 4.2–9.9 | 5.5 | | |
| Absolute Treg CD4+CD25+CD127− (cells/mcL) | 43–782 | 143 | | |
| Foxp3 (% of Tregs) | 55–81 | 32 | | |
| Absolute FOXP3 (cells/mcL) | 43–782 | 46 | | |
| Immunoglobulins | ||||
| IgG (mg/dl) | 100–334 | 205 | 501 | 906 |
| IgA (mg/dl) | 7–37 | 18 | | |
| IgM(mg/dl) | 26–122 | 19 | | |
| IgE (IU/ml) | <=13 | 9,309 | 4,997 | 31,478 |
| Serum cytokines | ||||
| IL-1 β (pg/ml) | <=6.7 | <6.5 | <6.5 | <6.5 |
| IL-2 (pg/ml) | <=2.1 | <2.1 | <2.1 | <2.1 |
| IL-2 receptor, soluble (pg/ml) | 175.3–858.2 | 6,111.5 | 3,787 | 2,322 |
| IL-4 (pg/ml) | <=2.2 | <2.2 | <2.2 | <2.2 |
| IL-5 (pg/ml) | <=2.1 | 6.1 | 2.2 | <2.1 |
| IL-6 (pg/ml) | <=2.0 | 49.6 | 2.8 | 8.1 |
| IL-8 (pg/ml) | <=3.0 | 278.1 | <3.0 | 8.4 |
| IL-10 (pg/ml) | <=2.8 | 48.7 | 10.6 | 10.6 |
| IL-12 (pg/ml) | <=1.9 | <1.9 | <1.9 | <1.9 |
| IL-13 (pg/ml) | <=2.3 | 2.9 | <1.7 | 2.3 |
| IL-17 (pg/ml) | <=1.4 | 1.5 | 44.7 | 12.8 |
| IL-18 (pg/ml) | <=477 | 1,685 | | |
| Interferon-γ (pg/ml) | <=4.2 | 5.6 | <4.2 | <4.2 |
| TNF-α (pg/ml) | <=7.2 | 5.4 | 2.2 | 3.8 |
| CXCL9 (pg/ml) | <=647 | 9,535 | | |
| C-reactive protein (CRP) (mg/dl) | <0.3 | 19.2 | 5.5 | 10.9 |
| Erythrocyte Sedimentation rate (ESR) (mm/h) | 0–9 | 14 | 20 | 4 |
| Procalcitonin (ng/ml) | 0–0.09 | 0.31 | | 14.6 |
| Fecal calprotectin (mcg/g) | | 457 | 1,200 | 1,380 |
| Fecal pancreatic elastase (ug/g) | > 200 | 10 | 36 | |
| Autoantibodies | ||||
| Glutamate decarboxylase antibody (IU/ml) | 0–5.0 | <5.0 (negative) | | |
| Islet antigen-2 serum autoantibody (unit/ml) | 0–7.4 | <5.4 (negative) | | |
| Insulin antibody (unit/ml) | 0–0.4 | <0.4 (negative) | | |
| Islet cell cytoplasmic antibody, IgG | <1:4 | <1:4 (negative) | | |
| Zinc transporter 8 antibody (unit/ml) | 0–15.0 | <10.0 (negative) | | |