Table 1. Summary of titin and cMyBP-C... | Rockefeller University Press

Table 1.

Summary of titin and cMyBP-C epitope distances

Epitope	WT distance (nm)	Ttn^ΔA164–167 distance (nm)	Difference (nm)	P value	Significance
M8–M10	41 ± 5	43 ± 6	2	0.5223	ns
A168–170 α	87 ± 6	70 ± 11	17	0.0473	*
A168–170 β	125 ± 6	-	-	-
cMyBP-C 1	165 ± 8	126 ± 13	39	0.0052	**
A77–78	443 ± 21	407 ± 21	37	0.0032	**
cMyBP-C 9	509 ± 11	463 ± 14	46	0.0057	**
Ti102	776 ± 33	736 ± 27	40	0.0097	**

Epitope	WT distance (nm)	Ttn^ΔA164–167 distance (nm)	Difference (nm)	P value	Significance
M8–M10	41 ± 5	43 ± 6	2	0.5223	ns
A168–170 α	87 ± 6	70 ± 11	17	0.0473	*
A168–170 β	125 ± 6	-	-	-
cMyBP-C 1	165 ± 8	126 ± 13	39	0.0052	**
A77–78	443 ± 21	407 ± 21	37	0.0032	**
cMyBP-C 9	509 ± 11	463 ± 14	46	0.0057	**
Ti102	776 ± 33	736 ± 27	40	0.0097	**

For each antibody, the measured distance from the center of the sarcomere (middle of M-band) to the epitope is listed for WT and Ttn^ΔA164–167 (±SD). The calculated difference between the WT and Ttn^ΔA164–167 distance is listed for each epitope, with statistical significance determined by a nested t test. The list is in order from closest to the M-line to farthest from the M-line. For titin epitopes in the distal part of the A-band (A77–78 and Ti102/MIR), we have only included data in the sarcomere length range of 1,700–2,300 nm. For titin epitopes more proximal to the M-band (A168–170 and M8–M10) and MyBP-C, data are from sarcomeres ranging in length from ∼2,000 to 2,600 nm. See Fig. S6 for epitope distance–sarcomere length relationships. ns, p ≥ 0.05; * p ≤ 0.05; ** p ≤0.01.

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