Relative permeability in NavAb Met181 mutants
| . | PLi/PNa . | PK/PNa . | PCs/PNa . | PCa/PNa . |
|---|---|---|---|---|
| N49K/T206A-M181A | 0.84 ± 0.03 | <0.05 | <0.05 | 0.36 ± 0.02 |
| (n = 8) | (n = 3) | (n = 6) | (n = 4) | |
| S178ANK/TA-M181A | 1.09 ± 0.04 | 0.09 ± 0.01 | <0.05 | 0.41 ± 0.05 |
| (n = 7; P = 2.2 × 10−3) | (n = 5) | (n = 5) | (n = 7; P = 0.63) | |
| S178GNK/TA-M181A (LivAbNK/TA-M181A) | 0.99 ± 0.04 | <0.05 | <0.05 | 0.47 ± 0.05 |
| (n = 3; P = 0.16) | (n = 3) | (n = 3) | (n = 4; P = 0.24) |
| . | PLi/PNa . | PK/PNa . | PCs/PNa . | PCa/PNa . |
|---|---|---|---|---|
| N49K/T206A-M181A | 0.84 ± 0.03 | <0.05 | <0.05 | 0.36 ± 0.02 |
| (n = 8) | (n = 3) | (n = 6) | (n = 4) | |
| S178ANK/TA-M181A | 1.09 ± 0.04 | 0.09 ± 0.01 | <0.05 | 0.41 ± 0.05 |
| (n = 7; P = 2.2 × 10−3) | (n = 5) | (n = 5) | (n = 7; P = 0.63) | |
| S178GNK/TA-M181A (LivAbNK/TA-M181A) | 0.99 ± 0.04 | <0.05 | <0.05 | 0.47 ± 0.05 |
| (n = 3; P = 0.16) | (n = 3) | (n = 3) | (n = 4; P = 0.24) |
All values are indicated as SEM. When the reversal potential was below the deactivation potential, PX/PNa ratios (X represents K, Cs, or Ca) were noted as <0.05, which is the value calculated from the lowest measurable reversal potential in each construct. Statistical significance obtained from Dunnett’s multiple comparison test. P values are the statistical significances compared with NavAb N49K/T206A-M181A. Statistical tests were not performed for comparisons where PX/PNa ratios were too low (<0.05).