Relative permeability in NavAb N49K/T206A, S178TNK/TA, S178ANK/TA, and S178GNK/TA mutants
| . | PLi/PNa . | PK/PNa . | PCs/PNa . | PCa/PNa . |
|---|---|---|---|---|
| N49K/T206A | 0.71 ± 0.02 | <0.05 | <0.05 | <0.05 |
| (n = 10) | (n = 6) | (n = 6) | (n = 8) | |
| S178TNK/TA | 0.96 ± 0.01 | 0.13 ± 0.01 | <0.05 | <0.05 |
| (n = 8; P = 0.17) | (n = 4) | (n = 7) | (n = 7) | |
| S178ANK/TA | 1.09 ± 0.05 | <0.05 | <0.05 | 0.12 ± 0.00 |
| (n = 11; P = 0.01) | (n = 8) | (n = 6) | (n = 6) | |
| S178GNK/TA (LivAbNK/TA) | 2.18 ± 0.14 | <0.05 | <0.05 | <0.05 |
| (n = 11; P = 3.3 × 10−16) | (n = 5) | (n = 7) | (n = 9) |
| . | PLi/PNa . | PK/PNa . | PCs/PNa . | PCa/PNa . |
|---|---|---|---|---|
| N49K/T206A | 0.71 ± 0.02 | <0.05 | <0.05 | <0.05 |
| (n = 10) | (n = 6) | (n = 6) | (n = 8) | |
| S178TNK/TA | 0.96 ± 0.01 | 0.13 ± 0.01 | <0.05 | <0.05 |
| (n = 8; P = 0.17) | (n = 4) | (n = 7) | (n = 7) | |
| S178ANK/TA | 1.09 ± 0.05 | <0.05 | <0.05 | 0.12 ± 0.00 |
| (n = 11; P = 0.01) | (n = 8) | (n = 6) | (n = 6) | |
| S178GNK/TA (LivAbNK/TA) | 2.18 ± 0.14 | <0.05 | <0.05 | <0.05 |
| (n = 11; P = 3.3 × 10−16) | (n = 5) | (n = 7) | (n = 9) |
All values are indicated as SEM. When the reversal potential was below the deactivation potential, PX/PNa ratios (X represents K, Cs, or Ca) were noted as <0.05, which is the value calculated from the lowest measurable reversal potential in each construct. Statistical significances are obtained from Dunnett’s multiple comparison test. P values are the statistical significances compared with NavAb N49K/T206A. Statistical tests were not performed for comparisons where PX/PNa ratios were too low (<0.05).