Common symptoms and root causes when working with permeabilized muscle experiments
| Common symptoms . | Possible root causes . |
|---|---|
| Ice crystals | Muscle samples larger than 1 cm in size |
| Low glycerol percentage for cyropreservation | |
| Samples not fully submersed in glycerol | |
| Damaged tissues | Dissection technique |
| Long preparation time | |
| Buffers (osmolarity, pH, and calcium) | |
| Poor permeabilization | Thick samples leading to a rigor core |
| Samples not fully submersed in detergent | |
| Suboptimal incubation times/protocol | |
| Mechanical measurement instabilities | Loose sample attachment (slippage) |
| Temperature drift | |
| Environment (room vibrations and EM interference) | |
| Poorly tuned force transducers/motors | |
| Loose epoxy, nuts, bolts, etc. |
| Common symptoms . | Possible root causes . |
|---|---|
| Ice crystals | Muscle samples larger than 1 cm in size |
| Low glycerol percentage for cyropreservation | |
| Samples not fully submersed in glycerol | |
| Damaged tissues | Dissection technique |
| Long preparation time | |
| Buffers (osmolarity, pH, and calcium) | |
| Poor permeabilization | Thick samples leading to a rigor core |
| Samples not fully submersed in detergent | |
| Suboptimal incubation times/protocol | |
| Mechanical measurement instabilities | Loose sample attachment (slippage) |
| Temperature drift | |
| Environment (room vibrations and EM interference) | |
| Poorly tuned force transducers/motors | |
| Loose epoxy, nuts, bolts, etc. |