Key resources table
| Materials and reagents . | Source . | Identifier . |
|---|---|---|
| Antibody | | |
| LR-ExM anti-FITC-phalloidin anti-mouse two color kit | Cytoseen | LR-RBF-MD-025 |
| G3BP1 antibody (TT-Y) | Santa Cruz Biotechnology | sc-81940 |
| Sec61b (D5Q1W) Rabbit mAb | Cell Signaling Technology | 14648 |
| Anti-TOMM20 antibody [EPR15581-54] | Abcam | ab186735 |
| LAMP-2 (H4B4) | Santa Cruz Biotechnology | sc-18822 |
| Anti-clathrin heavy chain antibody | Abcam | ab21679 |
| Fluorescein Phalloidin | Invitrogen | F432 |
| Fluorescein/Oregon Green Polyclonal Antibody, Alexa Fluor 488 | Invitrogen | A11096 |
| LR-ExM anti-rabbit antibody-digoxigenin trifunctional anchor and anti-digoxigenin dye kit | Cytoseen | LR-RD488-050 |
| LR-ExM anti-mouse antibody-digoxigenin trifunctional anchor and anti-digoxigenin dye kit | Cytoseen | LR-MD568-050 |
| AffiniPure Goat Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch | 111-005-144 |
| AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson ImmunoResearch | 115-005-166 |
| DyLight 405 Streptavidin | Jackson ImmunoResearch | 016-470-084 |
| Alexa Fluor 488 Streptavidin | Jackson ImmunoResearch | 016-540-084 |
| Protein and lipid labeling reagents | | |
| land-ExM NHS-biotin-MA and Streptavidin-488 kit | Cytoseen | LD-P488-050 |
| land-ExM NHS-biotin-MA and Streptavidin-405 kit | Cytoseen | LD-P405-050 |
| mCLING-Atto 647N | Synaptic Systems | 710 006AT647N |
| Alexa Fluor 488 NHS Ester (Succinimidyl ester) | Invitrogen | A20000 |
| Alexa Fluor 568 NHS Ester (Succinimidyl ester) | Invitrogen | A20003 |
| Other reagents and supplies | | |
| SYLGARD 184 Silicone Elastomer Kit | Ellsworth | 2137054 |
| 12-mm, no. 1 cover glass | Bellco Glass | 1943–10012A |
| Paraformaldehyde | Electron Microscopy Sciences | 50980488 |
| Formaldehyde | Sigma-Aldrich | F8775 |
| Aqueous glutaraldehyde EM Grade 10% | Electron Microscopy Sciences | 16120 |
| Triton X-100 | Sigma-Aldrich | 9002-93-1 |
| Bovine serum albumin | Thermo Fisher Scientific | BP9703100 |
| N-Succinimidyl methacrylate (NHS-MA) | Fisher Scientific | S08125G |
| Glycidyl methacrylate (GMA) | Sigma-Aldrich | 151238 |
| Sodium acrylate, 25 g | Santa Cruz Biotechnology | sc-236893 |
| Acrylamide | Sigma-Aldrich | A9099-100 G |
| N,N′-(1,2-Dihydroxyethylene)bisacrylamide | Sigma-Aldrich | 294381-5G |
| N,N′-Methylenebisacrylamide | Sigma-Aldrich | M7279-25 G |
| N,N,N′,N′-Tetramethylethylenediamine | Sigma-Aldrich | T9281-100 Ml |
| Ammonium persulfate | Sigma-Aldrich | A3678-25 G |
| Sodium Dodecyl Sulfate | Thermo Fisher Scientific | BP166-500 |
| 1 M Tris HCI Buffer, pH 7.5 | Invitrogen | 15567027 |
| Proteinase K | Sigma-Aldrich | P4850-5Ml |
| Materials and reagents . | Source . | Identifier . |
|---|---|---|
| Antibody | | |
| LR-ExM anti-FITC-phalloidin anti-mouse two color kit | Cytoseen | LR-RBF-MD-025 |
| G3BP1 antibody (TT-Y) | Santa Cruz Biotechnology | sc-81940 |
| Sec61b (D5Q1W) Rabbit mAb | Cell Signaling Technology | 14648 |
| Anti-TOMM20 antibody [EPR15581-54] | Abcam | ab186735 |
| LAMP-2 (H4B4) | Santa Cruz Biotechnology | sc-18822 |
| Anti-clathrin heavy chain antibody | Abcam | ab21679 |
| Fluorescein Phalloidin | Invitrogen | F432 |
| Fluorescein/Oregon Green Polyclonal Antibody, Alexa Fluor 488 | Invitrogen | A11096 |
| LR-ExM anti-rabbit antibody-digoxigenin trifunctional anchor and anti-digoxigenin dye kit | Cytoseen | LR-RD488-050 |
| LR-ExM anti-mouse antibody-digoxigenin trifunctional anchor and anti-digoxigenin dye kit | Cytoseen | LR-MD568-050 |
| AffiniPure Goat Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch | 111-005-144 |
| AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson ImmunoResearch | 115-005-166 |
| DyLight 405 Streptavidin | Jackson ImmunoResearch | 016-470-084 |
| Alexa Fluor 488 Streptavidin | Jackson ImmunoResearch | 016-540-084 |
| Protein and lipid labeling reagents | | |
| land-ExM NHS-biotin-MA and Streptavidin-488 kit | Cytoseen | LD-P488-050 |
| land-ExM NHS-biotin-MA and Streptavidin-405 kit | Cytoseen | LD-P405-050 |
| mCLING-Atto 647N | Synaptic Systems | 710 006AT647N |
| Alexa Fluor 488 NHS Ester (Succinimidyl ester) | Invitrogen | A20000 |
| Alexa Fluor 568 NHS Ester (Succinimidyl ester) | Invitrogen | A20003 |
| Other reagents and supplies | | |
| SYLGARD 184 Silicone Elastomer Kit | Ellsworth | 2137054 |
| 12-mm, no. 1 cover glass | Bellco Glass | 1943–10012A |
| Paraformaldehyde | Electron Microscopy Sciences | 50980488 |
| Formaldehyde | Sigma-Aldrich | F8775 |
| Aqueous glutaraldehyde EM Grade 10% | Electron Microscopy Sciences | 16120 |
| Triton X-100 | Sigma-Aldrich | 9002-93-1 |
| Bovine serum albumin | Thermo Fisher Scientific | BP9703100 |
| N-Succinimidyl methacrylate (NHS-MA) | Fisher Scientific | S08125G |
| Glycidyl methacrylate (GMA) | Sigma-Aldrich | 151238 |
| Sodium acrylate, 25 g | Santa Cruz Biotechnology | sc-236893 |
| Acrylamide | Sigma-Aldrich | A9099-100 G |
| N,N′-(1,2-Dihydroxyethylene)bisacrylamide | Sigma-Aldrich | 294381-5G |
| N,N′-Methylenebisacrylamide | Sigma-Aldrich | M7279-25 G |
| N,N,N′,N′-Tetramethylethylenediamine | Sigma-Aldrich | T9281-100 Ml |
| Ammonium persulfate | Sigma-Aldrich | A3678-25 G |
| Sodium Dodecyl Sulfate | Thermo Fisher Scientific | BP166-500 |
| 1 M Tris HCI Buffer, pH 7.5 | Invitrogen | 15567027 |
| Proteinase K | Sigma-Aldrich | P4850-5Ml |