Structural elucidation of the OSCA/TMEM63 protein family summary of high-resolution structures of OSCA/TMEM63 channels, revealing their oligomeric states, pore architecture, lipid interactions, and functional states with mechanistic hints
| Protein . | Species . | PDB . | Å . | Detergent . | Oligom-erization . | Functional state . | Ref . |
|---|---|---|---|---|---|---|---|
| OSCA1.1 | A. thaliana | 6JPF | 3.52 | MNG, CHS | Dimer | Closed state | Zhang et al. (2018) |
| OSCA1.1 | A. thaliana | 8GRN | 2.5 | LMNG, CHS | Dimer | Closed state in nanodiscs | Zhang et al. (2023a) |
| OSCA1.1-F516A | A. thaliana | 8YMO | 2.7 | LMNG, CHS | Dimer | Pre-open state 1 | Shan et al. (2024) |
| OSCA1.1-F516A | A. thaliana | 8YMN | 2.5 | LMNG, CHS | Dimer | Pre-open state 2 | Shan et al. (2024) |
| OSCA1.1-F516A | A. thaliana | 8YMM | 2.8 | LMNG, CHS | Dimer | Open state | Shan et al. (2024) |
| OSCA1.1-F516A | A, thaliana | 8YMQ | 2.6 | LMNG, CHS | Dimer | Closed state in nanodiscs | Shan et al. (2024) |
| OSCA1.1-F516A | A. thaliana | 8YMP | 2.6 | LMNG, CHS | Dimer | Nanodisc in LPC | Shan et al. (2024) |
| OSCA1.2 | A. thaliana | 6MGV | 3.1 | LMNG, CHS | Dimer | Closed state in nanodiscs | Jojoa-Cruz et al. (2018) |
| OSCA1.2 | A, thaliana | 6MGW | 3.5 | LMNG, CHS | Dimer | Closed state | Jojoa-Cruz et al. (2018) |
| OSCA1.2 | A. thaliana | 6IJZ | 3.68 | GDN | Dimer | Closed state | Liu et al. (2018) |
| OSCA1.2 | Oryza sativa | 6OCE | 4.9 | DDM, sodium cholate | Dimer | Closed state | Maity et al. (2019) |
| OSCA1.2 | A. thaliana | 8T56 | 2.8 | DDM, CHS | Dimer | Closed state in Peptidiscs | Jojoa-Cruz et al. (2024a) |
| OSCA1.2 | A. thaliana | 8XS4 | 3.23 | DDM, CHS | Dimer | Contracted state 1: MSP:DOPC = 1:20 in nanodisc | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XS5 | 3.33 | DDM, CHS | Dimer | Contracted state 2: MSP:DOPC = 1:20 in nanodisc | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XNG | 3.56 | DDM, CHS | Dimer | Closed state: Liposome-inside-out | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XAJ | 3.29 | DDM, CHS | Dimer | Open state:liposome-inside-in | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XW2 | 3.59 | DDM, CHS | Dimer | Contracted state: MSP:DOPC = 1:50 in nanodisc | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XW3 | 3.63 | DDM, CHS | Dimer | Expanded state: MSP:DOPC = 1:50 in nanodisc | Han et al. (2024) |
| OSCA1.2-V335W | A. thaliana | 8XW1 | 4.49 | DDM, CHS | Monomer | Closed state | Han et al. (2024) |
| OSCA2.3 | A. thaliana | 8T57 | 2.7 | DDM, CHS | Dimer | Closed state in Peptidiscs | Jojoa-Cruz et al. (2024a) |
| OSCA3.1 | A. thaliana | 5Z1F | 4.8 | MNG, CHS | Dimer | Closed state | Zhang et al. (2018) |
| OSCA3.1 | A. thaliana | 8GSO | 3.3 | LMNG, CHS | Dimer | Expanded state | Zhang et al. (2023a) |
| OSCA3.1 | A. thaliana | 8GRO | 3.5 | LMNG, CHS | Dimer | Contracted state | Zhang et al. (2023a) |
| OSCA3.1 | A. thaliana | 8U53 | 2.6 | LMNG, CHS | Dimer | Closed state in nanodiscs | Jojoa-Cruz et al. (2024b) |
| OSCA3.1 | A. thaliana | 8XW0 | 3.11 | GDN | Dimer | Closed state | Han et al. (2024) |
| OSCA3.1-Y367N-G454S-Y458I | A. thaliana | 8XRY | 3.84 | GDN | Dimer | Open/open state | Han et al. (2024) |
| OSCA3.1-Y367N-G454S-Y458I | A. thaliana | 8XS0 | 3.89 | GDN | Dimer | Open/desensitized state | Han et al. (2024) |
| OSCA3.1-R611E-R619E | A. thaliana | 8XVY | 3.71 | GDN | Dimer | Closed/open state | Han et al. (2024) |
| OSCA3.1-R611E-R619E | A. thaliana | 8XVZ | 3.78 | GDN | Dimer | Closed/desensitized state | Han et al. (2024) |
| TMEM63A | Homo sapiens | 8GRS | 3.3 | LMNG, CHS | Monomer | Closed state | Zhang et al. (2023a) |
| TMEM63A | Homo sapiens | 8EHW | 3.8 | LMNG, CHS | Monomer | Closed state in nanodiscs | Zheng et al. (2023) |
| TMEM63A | Homo sapiens | 8WUA | 3.6 | DDM, CHS | Monomer | Closed state | Wu et al. (2024) |
| TMEM63A-V53M | Homo sapiens | 9N95 | 3.65 | LMNG, CHS | Monomer | Closed state | Zheng et al. (2025) |
| TMEM63A-V53M | Homo sapiens | 9N93 | 2.95 | LMNG, CHS | Monomer | Lipid open state | Zheng et al. (2025) |
| TMEM63B | Homo sapiens | 8EHX | 3.6 | LMNG, CHS | Monomer | Closed state | Zheng et al. (2023) |
| TMEM63B | Homo sapiens | 8XW4 | 3.84 | GDN | Monomer | Closed state | Han et al. (2024) |
| TMEM63B | Mus musculus | 8WG3 | 3.4 | LMNG, CHS | Monomer | Closed state | Miyata et al. (2024) |
| TMEM63B | Mus musculus | 8WG4 | 3.5 | DDM, CHS | Monomer | Lipid open state with YN9303-24 | Miyata et al. (2024) |
| TMEM63C | Mus musculus | 8K0B | 3.56 | LMNG, CHS | Monomer | Closed state | Qin et al. (2023) |
| Protein . | Species . | PDB . | Å . | Detergent . | Oligom-erization . | Functional state . | Ref . |
|---|---|---|---|---|---|---|---|
| OSCA1.1 | A. thaliana | 6JPF | 3.52 | MNG, CHS | Dimer | Closed state | Zhang et al. (2018) |
| OSCA1.1 | A. thaliana | 8GRN | 2.5 | LMNG, CHS | Dimer | Closed state in nanodiscs | Zhang et al. (2023a) |
| OSCA1.1-F516A | A. thaliana | 8YMO | 2.7 | LMNG, CHS | Dimer | Pre-open state 1 | Shan et al. (2024) |
| OSCA1.1-F516A | A. thaliana | 8YMN | 2.5 | LMNG, CHS | Dimer | Pre-open state 2 | Shan et al. (2024) |
| OSCA1.1-F516A | A. thaliana | 8YMM | 2.8 | LMNG, CHS | Dimer | Open state | Shan et al. (2024) |
| OSCA1.1-F516A | A, thaliana | 8YMQ | 2.6 | LMNG, CHS | Dimer | Closed state in nanodiscs | Shan et al. (2024) |
| OSCA1.1-F516A | A. thaliana | 8YMP | 2.6 | LMNG, CHS | Dimer | Nanodisc in LPC | Shan et al. (2024) |
| OSCA1.2 | A. thaliana | 6MGV | 3.1 | LMNG, CHS | Dimer | Closed state in nanodiscs | Jojoa-Cruz et al. (2018) |
| OSCA1.2 | A, thaliana | 6MGW | 3.5 | LMNG, CHS | Dimer | Closed state | Jojoa-Cruz et al. (2018) |
| OSCA1.2 | A. thaliana | 6IJZ | 3.68 | GDN | Dimer | Closed state | Liu et al. (2018) |
| OSCA1.2 | Oryza sativa | 6OCE | 4.9 | DDM, sodium cholate | Dimer | Closed state | Maity et al. (2019) |
| OSCA1.2 | A. thaliana | 8T56 | 2.8 | DDM, CHS | Dimer | Closed state in Peptidiscs | Jojoa-Cruz et al. (2024a) |
| OSCA1.2 | A. thaliana | 8XS4 | 3.23 | DDM, CHS | Dimer | Contracted state 1: MSP:DOPC = 1:20 in nanodisc | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XS5 | 3.33 | DDM, CHS | Dimer | Contracted state 2: MSP:DOPC = 1:20 in nanodisc | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XNG | 3.56 | DDM, CHS | Dimer | Closed state: Liposome-inside-out | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XAJ | 3.29 | DDM, CHS | Dimer | Open state:liposome-inside-in | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XW2 | 3.59 | DDM, CHS | Dimer | Contracted state: MSP:DOPC = 1:50 in nanodisc | Han et al. (2024) |
| OSCA1.2 | A. thaliana | 8XW3 | 3.63 | DDM, CHS | Dimer | Expanded state: MSP:DOPC = 1:50 in nanodisc | Han et al. (2024) |
| OSCA1.2-V335W | A. thaliana | 8XW1 | 4.49 | DDM, CHS | Monomer | Closed state | Han et al. (2024) |
| OSCA2.3 | A. thaliana | 8T57 | 2.7 | DDM, CHS | Dimer | Closed state in Peptidiscs | Jojoa-Cruz et al. (2024a) |
| OSCA3.1 | A. thaliana | 5Z1F | 4.8 | MNG, CHS | Dimer | Closed state | Zhang et al. (2018) |
| OSCA3.1 | A. thaliana | 8GSO | 3.3 | LMNG, CHS | Dimer | Expanded state | Zhang et al. (2023a) |
| OSCA3.1 | A. thaliana | 8GRO | 3.5 | LMNG, CHS | Dimer | Contracted state | Zhang et al. (2023a) |
| OSCA3.1 | A. thaliana | 8U53 | 2.6 | LMNG, CHS | Dimer | Closed state in nanodiscs | Jojoa-Cruz et al. (2024b) |
| OSCA3.1 | A. thaliana | 8XW0 | 3.11 | GDN | Dimer | Closed state | Han et al. (2024) |
| OSCA3.1-Y367N-G454S-Y458I | A. thaliana | 8XRY | 3.84 | GDN | Dimer | Open/open state | Han et al. (2024) |
| OSCA3.1-Y367N-G454S-Y458I | A. thaliana | 8XS0 | 3.89 | GDN | Dimer | Open/desensitized state | Han et al. (2024) |
| OSCA3.1-R611E-R619E | A. thaliana | 8XVY | 3.71 | GDN | Dimer | Closed/open state | Han et al. (2024) |
| OSCA3.1-R611E-R619E | A. thaliana | 8XVZ | 3.78 | GDN | Dimer | Closed/desensitized state | Han et al. (2024) |
| TMEM63A | Homo sapiens | 8GRS | 3.3 | LMNG, CHS | Monomer | Closed state | Zhang et al. (2023a) |
| TMEM63A | Homo sapiens | 8EHW | 3.8 | LMNG, CHS | Monomer | Closed state in nanodiscs | Zheng et al. (2023) |
| TMEM63A | Homo sapiens | 8WUA | 3.6 | DDM, CHS | Monomer | Closed state | Wu et al. (2024) |
| TMEM63A-V53M | Homo sapiens | 9N95 | 3.65 | LMNG, CHS | Monomer | Closed state | Zheng et al. (2025) |
| TMEM63A-V53M | Homo sapiens | 9N93 | 2.95 | LMNG, CHS | Monomer | Lipid open state | Zheng et al. (2025) |
| TMEM63B | Homo sapiens | 8EHX | 3.6 | LMNG, CHS | Monomer | Closed state | Zheng et al. (2023) |
| TMEM63B | Homo sapiens | 8XW4 | 3.84 | GDN | Monomer | Closed state | Han et al. (2024) |
| TMEM63B | Mus musculus | 8WG3 | 3.4 | LMNG, CHS | Monomer | Closed state | Miyata et al. (2024) |
| TMEM63B | Mus musculus | 8WG4 | 3.5 | DDM, CHS | Monomer | Lipid open state with YN9303-24 | Miyata et al. (2024) |
| TMEM63C | Mus musculus | 8K0B | 3.56 | LMNG, CHS | Monomer | Closed state | Qin et al. (2023) |
MNG, maltose neopentyl glycol; CHS, cholesteryl hemisuccinate; LMNG, lauryl maltose neopentyl glycol; DDM, n-dodecyl-β-D-maltopyranoside; GDN, digitonin; DOPC, dioleoylphosphatidylcholine; MSP, membrane scaffold protein.