Table 1.

Summary of the dominant negative effect of ADA2 variants on WT ADA2 dimer secretion, and on intracellular and extracellular ADA2 enzymatic activity

VariantDimer secretionIntracellular ADA2 enzymatic activityExtracellular ADA2 enzymatic activity
G47A − 
G47R − 
G47V 
G47W − − − 
R169Q ++ ++ 
E328K − 
F355L − − − 
T360A − − − 
N370K − − − 
H424N ++ ++ ++ 
Y453C ++ ++ ++ 
VariantDimer secretionIntracellular ADA2 enzymatic activityExtracellular ADA2 enzymatic activity
G47A − 
G47R − 
G47V 
G47W − − − 
R169Q ++ ++ 
E328K − 
F355L − − − 
T360A − − − 
N370K − − − 
H424N ++ ++ ++ 
Y453C ++ ++ ++ 

−: no dominant negative effect; +: noticeable dominant negative effect; ++: pronounced dominant negative effect.

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